山东医药2017，Vol.57Issue(37)：15-17,3.DOI:10.3969/j.issn.1002-266X.2017.37.005
姜黄素对宫颈癌干细胞增殖、聚集及化疗敏感性的影响
Effects of curcumin on cell proliferation, aggregation, and chemosensitivity of cervical cancer stem cells
摘要
Abstract
Objective To explore the effects of curcumin on the cell proliferation,aggregation,and the sensitivity to bevacizumab of cervical cancer stem cells.Methods The CD133 + cervical cancer stem cells (CCSCs) were isolated from CASKI cells by flow cytometry.The CCSCs were divided into seven groups:CC10 group,CC20 group,CC40 group,CC60 group,CC80 group,and CC100 group which were given a final concentration of 10,20,40,60,80 and 100 mg/mL curcumin,while cells in the control group were given the same amount of solvent and culture medium.The inhibition rate of cell proliferation in each group was detected by MTF.The cells in the control group and CC60 group were cultured in serum-free medium for 1 week.The number of stem cell spheres with diameter greater than 100 μm was counted by light microscope,and the proportion of CD133 + cells was detected by flow cytometry.The cervical cancer stem cells were divided into four groups,BV group (added with bevacizumab 10 mg/mL),CC group (added with 60 mg/mL curcumin),BV + CC group (added with 10 mg/mL bevacizumab and 60 mg/mL curcumin),and the control group (treated with the same amount of solvent and medium).After 48-hour culture,the apoptosis rate was determined by flow cytometry.Results The rates of cell proliferation inhibition in the CC10 group,CC20 group,CC40 group,CC60 group,CC80 group,and CC100 group were higher than that of the control group (all P ＜ 0.05),and with the increase of curcumin concentration,the proliferation inhibition rate of cervical cancer stem cells gradually increased (all P ＜ 0.05).The number of stem cell spheres with diameter greater than 100μam of the CC60 group was less than that of the control group (P ＜ 0.05).The proportion of cervical cancer stem cell in the CC60 group was lower than that in the control group (P ＜ 0.05).The apoptosis rate in the BV + CC group was higher than that in the control group,BV group,and CC group (P ＜ 0.05),the apoptosis rate in the CC group was higher than that in the control group and BV group (P ＜ 0.05),and the apoptosis rate in the BV group was higher than that in the control group (P ＜ 0.05).Conclusion Curcumin can inhibit the proliferation and decrease the stem cell aggregation of CCSCs and increase the sensitivity of CCSCs to bevacizumab.关键词
宫颈癌/宫颈癌干细胞/姜黄素/细胞增殖/细胞聚集/贝伐单抗/治疗敏感性Key words
cervical carcinoma/cervical cancer stem cells/curcumin/cell proliferation/cell aggregation/bevacizumab/treatment sensitivity分类
医药卫生引用本文复制引用
张燕华,高艳娥,臧睿,杨红梅..姜黄素对宫颈癌干细胞增殖、聚集及化疗敏感性的影响[J].山东医药,2017,57(37):15-17,3.基金项目
陕西省自然科学基金资助项目(2016JM8067). （2016JM8067）
