山东医药2017，Vol.57Issue(37)：18-20,3.DOI:10.3969/j.issn.1002-266X.2017.37.006
远华蟾毒精对小鼠乳腺癌细胞侵袭的抑制作用及机制
Inhibitory effect of Yuanhua toad poison fine on invasion of mouse breast cancer cells
摘要
Abstract
Objective To investigate the inhibitory effect and mechanism of Yuanhua toad poison fine on the invasion of mouse breast cancer cells.Methods The cultured mouse breast cancer cells 4T1 were randomly divided into groups A,B,C,and D.Cell in the group A received the conventional culture;cells in the groups B,C,and D were cultured under the hypoxic conditions (at 37 ℃,5％ CO and 95％ N2);while cells in the groups C and D were added with 0.1 and 0.5 mol/L Yuanhua toad poison fine culture.At 0,6,12,and 24 h,the cell migration ability was examined by Scratch test (migration distance);the invasive ability was detected by Transwell invasion test (Number of cells through die);the relative expression levels of HIF-1α,matrix metalloproteinase 9 (MMP-9),phosphoinositide 3-kinase (PI3K),and p-Akt protein were detected by Western blotting.Results The migration distance of each group at different time points was in the following order:group D ＜ group C ＜ group B ＜ group A,the number of transmembrane cells:group D ＜ group C ＜group B ＜ group A,the expression of HIF-1α,MMP-9,p-PI3K,and p-Akt protein:group D ＜ group C ＜ group B ＜group A,and the differences in the above indexes were statistically significant between these two groups (all P ＜ 0.05).Conclusion Yuanhua toad poison fine can decrease the expression of HIF-1α and MMP-9 through the PI3K/AKT pathway,thereby inhibiting the invasion of breast cancer cells.关键词
乳腺癌/远华蟾毒精/细胞侵袭/4T1细胞/中药Key words
breast carcinoma/Yuanhua toad poison fine/cell invasion/4T1 cells/traditional Chinese medicine分类
医药卫生引用本文复制引用
曹珍,高玉雪,徐金媛,王茹燕,李峰,朱学涛,史立宏,吕世军..远华蟾毒精对小鼠乳腺癌细胞侵袭的抑制作用及机制[J].山东医药,2017,57(37):18-20,3.基金项目
山东省自然科学基金资助项目(H1622). （H1622）
