生物技术通报2018，Vol.34Issue(1)：104-109,6.DOI:10.13560/j.cnki.biotech.bull.1985.2017-0673
木薯叶片显微结构及叶绿素荧光参数比较
Comparison on Microstructure and Chlorophyll Fluorescence Parameter in Cassava
摘要
Abstract
In this study,wild cassava(Manihot glaziovii),wild relative W14(M. esculenta ssp. flabellifolia)and SC205(M. esculenta cv. SC No.205)were used as materials. Leaf anatomical structures and chlorophyll fluorescence parameter were analyzed in order to show the difference in its structure and photosynthetic activity. The microstructures of cassava functional leaves were observed by paraffin sections,95％ ethanol extraction method to extract chlorophyll directly,using PAM-2500 to measure chlorophyll fluorescence kinetics parameters of light systemII(PS II). Result showed that wild cassava and SC205 leaves were with "unique" green bundle sheath cell structure,but no typical"kranz structure"of C4 plant. The overall shape of green bundle sheath cells in W14 was slightly like the"kranz structure",but was not typical as C4 plant,while mesophyll cells were adjacent to the external of the green bundle sheath and they were arranged at slightly loose. Content of chlorophyll A and total chlorophyll in W14 leaves were significantly lower than wild cassava and SC205. Chlorophyll fluorescence parameter ΦPS II in them can be sequenced as SC205 > wild cassava> W14 ;however,Fv/Fm in W14 was higher than wild cassava and SC205,speculated that W14 might have a greater potential of photosynthetic capacity. Wild cassava and SC205 belong to C3 plant,W14 was a kind of cassava germplasm between C3 and C4. Their photosynthetic efficiencies in ΦPS II were in descending order of SC205,wild cassava,and W14.关键词
木薯/叶片/显微结构/叶绿素荧光/光合效率Key words
Manihot esculenta Crantz/leaves/microstructure/chlorophyll fluorescence/photosynthetic efficiency引用本文复制引用
安飞飞,陈霆,杨龙,陈松笔..木薯叶片显微结构及叶绿素荧光参数比较[J].生物技术通报,2018,34(1):104-109,6.基金项目
国家自然科学基金-青年基金项目(31601361),国家自然科学基金-国际交流与合作项目(31361140366),中国热带农业科学院热带作物品种资源研究所基本科研业务费(1630032016008) （31601361）
