生物技术通报2018，Vol.34Issue(1)：129-136,8.DOI:10.13560/j.cnki.biotech.bull.1985.2017-0746
铁皮石斛转化酶抑制子家族基因的克隆和表达分析
Cloning and Expression Analysis of Invertase Inhibitor Gene Families from Dendrobium officinale
摘要
Abstract
Invertase inhibitor(InvInh)controls the activity of invertase and plays a significant role in the sucrose metabolic processes. This study aims to clone member of InvInh gene in a rare and endangered medicinal plant Dendrobium officinale,and to quantitatively detect the expression in different organs and to conduct bioinformatics analysis. As results,three InvInh genes were cloned,named as DoInvInh1, DoInvInh2,and DoInvInh3,and they encoded 176,177,and 177 amino acids,respectively. Bioinformatics analysis of 3 DoInvInh proteins showed that there were 1 signal peptide,2 transmembrane structures,and the conservative domain structure of PMEI protein. Though the homology of InvInh protein amino acid sequence was very low among different species,there were 4 conservative Cys and SP sites in all of them. The results of quantitative real-time PCR demonstrated that the expressions of all 3 DoInvInh genes were detected in the roots,stems,leaves, and flowers. The DoInvInh2 and DoInvInh3 genes expressed the lowest in roots,while the highest in flowers. The DoInvInh1 and DoInvInh3 presented a similar gene expression pattern in stems at different growth years of D. officinale,i.e.,the highest expression in the stems of one year,the medium in the stems of two years,and the lowest in stems of three years. The DoInvInh2 gene was at the highest expression in the stems of two years. The expressions of DoInvInh1 and DoInvInh3 genes were significantly in negative correlation with CIN activities,speculated that two genes probably played regulatory roles in CIN activities.关键词
铁皮石斛/转化酶抑制子/基因克隆/基因表达Key words
Dendrobium officinale/invertase inhibitor/gene cloning/gene expression引用本文复制引用
苗小荣,牛俊奇,莫昭展,王爱勤,何龙飞..铁皮石斛转化酶抑制子家族基因的克隆和表达分析[J].生物技术通报,2018,34(1):129-136,8.基金项目
广西科学研究与技术开发计划项目(桂科重1298001-3-1),广西省南宁市重点研发项目(20162094) （桂科重1298001-3-1）
