生物技术通报2018，Vol.34Issue(1)：202-207,6.DOI:10.13560/j.cnki.biotech.bull.1985.2017-0630
一株解淀粉芽孢杆菌生防蛋白的鉴定及分析
Identification and Analysis of Biocontrol Proteins from a Strain of Bacillus amyloliquefaciens
摘要
Abstract
It is aimed at the identification of protein composition with biocontrol function in the secretory protein while a strain of being antagonistic grape downy mildew,Bacillus amyloliquefaciens,was cultured in NA liquid medium,which was carried out by protein profile. The proteomics of the identified proteins was analyzed by gene ontology and KEGG pathway for screening the proteins related to biocontrol. The results showed that 53 proteins were identified from the secreted proteins. Their relative molecular weights were mostly(79.24％)in 0-100. The proteins involved in the processes of carbohydrate metabolism,energy metabolism,lipid metabolism,amino acid metabolism,bio-defense,etc. They also were the cellular components in structural composition. Six proteins involved in the interaction of plant and pathogen were discovered,and among them,two belonged to the aminopeptidase family,and four were involved in the biodegradation of chitin.关键词
解淀粉芽孢杆菌/生物防治/基因本体论/氨基肽酶/几丁质酶Key words
Bacillus amyloliquefaciens/biocontrol/gene ontology/aminopeptidase/chitinase引用本文复制引用
郭继平,马光,王志杰,齐善厚,王宝梅,苏长青..一株解淀粉芽孢杆菌生防蛋白的鉴定及分析[J].生物技术通报,2018,34(1):202-207,6.基金项目
河北省青年拔尖人才支持计划项目(201619),河北省高等学校青年拔尖人才研究计划项目(BJ201608) （201619）
