生物技术通报2018，Vol.34Issue(1)：230-238,9.DOI:10.13560/j.cnki.biotech.bull.1985.2017-0728
连作对大豆根际土壤细菌菌群结构的影响
Effects of Continuous Cropping on Bacterial Flora Structure in Soybean Rhizosphere Soil
摘要
Abstract
The flora structure of soil bacteria is an important part of soil ecological environment quality. In order to find out the changes of bacterial flora structure in the rhizosphere soil after continuous cropping of soybean,soybean rhizosphere soil samples of zero- and 2-year of continuous cropping were selected,and their bacterial flora structures were studied using Illumina high-throughput sequencing technique. The results showed that the bacterial abundances and diversity indexes in the rhizosphere soil of zero-year continuous cropping were higher than those of the rhizosphere soil of 2-year continuous cropping. The abundances of these beneficial bacteria Cytophagaceae,Sphingomonas, Bradyrhizobium,and Streptomyces varied insignificantly. Soybean continuous cropping reduced the bacterial diversity of the soil and changed the bacterial flora structure. However,during the short-term continuous cropping process,the variation pattern of rhizosphere microbes in different crops differed,thus it was difficult to infer a common phenomenon resulted from continuous cropping. The results are aimed to provide a theoretical basis for alleviating the short-term soybean continuous cropping obstacle and increasing the yield of soybean.关键词
连作/大豆/根际土壤/细菌群落/Illumina高通量测序Key words
continuous cropping/soybean/rhizosphere soil/bacterial flora/Ilumina high-throughput sequencing引用本文复制引用
殷继忠,李亮,接伟光,蔡柏岩..连作对大豆根际土壤细菌菌群结构的影响[J].生物技术通报,2018,34(1):230-238,9.基金项目
国家自然科学基金面上项目(31570487),黑龙江东方学院横向科研课题重点项目(HDFHX160103) （31570487）
