特产研究2017，Vol.39Issue(4)：6-12,7.DOI:10.16720/j.cnki.tcyj.2017.04.002
仙茅组培快繁技术研究
Study on Rapid Propagation via Tissue Culture of Curculigo
摘要
Abstract
Callus was induced by different hormone concentrations in young leaves of Curculigo orchiodes.The suitable medium was selected to induce adventitious buds,and then the rooting experiment was carried out and transplanted to soil.The results showed that MS+2,4-D0.1 mg/L+KT1.5mg/L+carbendazim 3％+citric acid 8mg/L was the best in MS medium.Up to 96％.The subculture medium was MS+NAA0.3mg/L+6-BA2mg/L+2,4-D0.3mg/L,the best subculture was the MS,the highest callus quality was the best,More easily induced adventitious buds.Adding 0.3mg/L NNA to the medium was beneficial to rooting,and the survival rate was 90％ when transplanted into the nutrient soil.关键词
仙茅/组织培养/不定芽/移栽Key words
Curculigo orchiodes/tissue culture/adventitious bud/transplanting分类
农业科技引用本文复制引用
张虹,何钢,刘贤桂,梁文斌,曾柏全..仙茅组培快繁技术研究[J].特产研究,2017,39(4):6-12,7.基金项目
国家林业公益性行业科技专项项目(201304807) （201304807）
