特产研究2017，Vol.39Issue(4)：25-27,3.DOI:10.16720/j.cnki.tcyj.2017.04.006
牛巴泰病毒抗体间接ELISA检测方法的建立及应用
Development and Application of an Indirect ELISA Method for Detection of Antibody to Cattle Batai Virus
摘要
Abstract
The indirect ELISA with one strain NM/12 of Batai virus (BATV) as diagnostic antigen was established and optimized to detect antibodies against BATV in cattle sera.The result show that the established method were specific to detect anti-sera against BATV,but no cross-reaction to the sera against Akabane virus and other two kinds of common cattle virus.The sensitivity test showed that the positive serum were still positive in 1:640 times of dilution,which showed high sensitivity of the assay.The indirect ELISA was used to test against 523 serum samples collected from some areas of Inner Mongolia,Heilongjiang and Jilin provinces,and 10.71％ of samples were antibody-positive to BATV.Comparison test analysis showed that the compliance rate was 100％ between ELISA and neutralization test,based on the titration of 523 serum samples.All these indicated that the indirect ELISA is not only sensitive and specific,but also provide a simple and rapid method of detectin BATV infection.关键词
牛巴泰病毒/间接酶联免疫吸附试验/血清流行病学Key words
Cattle batai virus/indirect ELISA/epidemiology分类
农业科技引用本文复制引用
刘昊,胡博,张蕾,李欣彤,邓效禹,闫喜军..牛巴泰病毒抗体间接ELISA检测方法的建立及应用[J].特产研究,2017,39(4):25-27,3.基金项目
吉林省科技发展计划项目(20150520127JH) （20150520127JH）
中央级公益性科研院所基本科研业务费专项(1610342016034) （1610342016034）
