特产研究2017，Vol.39Issue(4)：40-46,7.DOI:10.16720/j.cnki.tcyj.2017.04.010
端粒及端粒酶活性检测方法研究进展
The Method and Progress of Telomerase Activity Detection
摘要
Abstract
Telomere shortening is the signal of cell senescence.Detection of telomerase activity can indirectly determine cell state.Telomerase activity affect permanent stagnation of embryonic development by p66Shc.Herein,relationship between telomerase activity and permanently arrest of mammalian oocytes or embryos and detection methods oftelomerase activity,the advantage and disadvantage ofvario us methods are reviewed,including TRAP,TRAP-ELISA,TRAP-silver stainined assay,HCR chain reaction signal amplification combined with magnetic separation technology and Methylene blue as G-quadruplex binding probe,Hemin-graphene conjugational colorimetric method,Label-free molecular beacons-based cascade amplification DNA machine.关键词
端粒/端粒酶/永久性胚胎发育阻滞/活性检测Key words
Telomere/Telomerase/permanent embryo arrest/activity detection分类
生物科学引用本文复制引用
张晓娜,杨镒峰,许保增..端粒及端粒酶活性检测方法研究进展[J].特产研究,2017,39(4):40-46,7.基金项目
中国农业科学院科技创新工程(CAAS-ASTIP-2017-ISAPS) （CAAS-ASTIP-2017-ISAPS）
吉林特研生物技术有限责任公司研发基金 （）
