天津中医药2018，Vol.35Issue(1)：41-44,4.DOI:10.11656/j.issn.1672-1519.2018.01.12
非索非那定体内探针药物法考察脑心通胶囊对大鼠P-gp活性的影响
Modulation of rat P-gp by multiple doses of Naoxintong capsule using fexofenadine as a phenotyping probe
摘要
Abstract
[Objective] A liquid chromatography tandem mass spectrometry method (LC-MS/MS) was developed and validated for the determination of fexofenadine in rats plasma, and the effects of Naoxintong on P-gp in rats were studied. [Methods] The samples were separated on a Waters XBridgeTM C18 column (2.1 mm×100 mm, 3.5μm) with a gradient mobile phase system of 0.1％(V/V) formic acid and acetonitrile . Fexofenadine and diphenhydramine ( Internal Standard ) were detected by electrospray ionization (ESI) with multiple reaction monitoring. The transitions at m/z 502.3→466.3 (fexofenadine), and m/z 256.1→166.9 (IS), respectively. [Results] A good linear response was observed over the concentration ranges of 1-250 ng/mL. Both intra and inter-day precision (RSD) were within 8.7％, and the extraction recovery were more than 83.0％. [Conclusion] The results suggested that the method proved to be highly reproducible and sensitive that was successfully applied in the pharmacokinetic study of fexofenadine, and the inhibitory effect of Naoxintong capsule on the activity of P-gp in rats after continuous administration of Naoxintong were observed.关键词
液相色谱-质谱联用法/非索非那定/脑心通/P-糖蛋白Key words
LC-MS/MS/fexofenadine/Naoxintong/P-gp分类
医药卫生引用本文复制引用
欧阳慧子,刘华明,王兴蕊,常艳旭,何俊..非索非那定体内探针药物法考察脑心通胶囊对大鼠P-gp活性的影响[J].天津中医药,2018,35(1):41-44,4.基金项目
国家自然科学基金项目(81503457) （81503457）
科技部国际合作项目(2015DFA30430). （2015DFA30430）
