天津中医药2018，Vol.35Issue(1)：68-71,4.DOI:10.11656/j.issn.1672-1519.2018.01.18
心脑舒通胶囊对血管性痴呆大鼠的神经保护作用
Neuroprotective effects of Xinnao Shutong capsule on vascular dementia in rats
摘要
Abstract
[Objective] To investigate the neuroprotective effect of Xinnao Shutong capsules ( XNST ) on vascular dementia in rats . [Methods] Adult male Wistar rats [weight (300±20) g, eleven weeks old] were used in this experiment. The rats were randomly divided into control group , model , and two treatment ( 14 , 56 mg/kg XNST ) groups . The model of vascular dementia in rats was induced by bilateral common carotid artery ligation (BCCAL). Then throughout a period of two months. XNST was given intragastrically to rats. This was followed by rats' behaviors evaluation by Morris water maze method to obtain records of their performance regarding learning and memory. The changes of brain cell morphology were observed by Nissl stain. The expression IBA-1and GFAP of the hippocampus were measured with immunohistochemistry staining. [Results] XNST improved the ability of learning and memory in vascular dementia rats and prevented the loss of pyramidal cells on CA1 zone in the hippocampus. Moreover, the neuronal cell number was increased dramatically after XNST treatment in dose dependent manner. The number of pyramidal cells in XNST increased significantly than that in model group (P<0.01). XNST (56 mg/kg) can the activation of microglia (IBA-1)expression (P<0.01). XNST (56 mg/kg) could obviously inhibit the activation of astrocytes (GFAP)expression (P<0.01). [Conclusion] XNST can improve the learning and memory ability of vascular dementia rats, the underlying mechanism of the effects may be related to its action of preventing neuronal cell of hippocampus against ischemic injury inhibiting the astrocytes and microglia activation.关键词
心脑舒通胶囊/血管性痴呆/Morris水迷宫/星形胶质细胞/小胶质细胞/神经Key words
Xinnao Shutong capsule/cascular dementia/Morris water maze/astrocyte/microglia/nerve分类
医药卫生引用本文复制引用
Batnairamdal Chuluun,王金鑫,李芮琳,卢国彦,郭虹,柴丽娟,张玥,王少峡,胡利民..心脑舒通胶囊对血管性痴呆大鼠的神经保护作用[J].天津中医药,2018,35(1):68-71,4.基金项目
国家重大新药创制项目(2011ZX09201201-033) （2011ZX09201201-033）
国家自然科学基金项目(8157140605). （8157140605）
