西北国防医学杂志2017，Vol.38Issue(12)：783-787,5.DOI:10.16021/j.cnki.1007-8622.2017.12.004
不同液体复苏对重度失血性休克大鼠肝脏损伤的影响
Effects of different fluids resuscitation on liver injury in rats with severe hemorrhagic shock
摘要
Abstract
Objective:To obeserve the effects of different fluids resuscitation on liver injury in rats with severe hemorrhagic shock.Methods:Sixty SD rats were randomly divided into 5 groups:control group(group C),LR treated group(group LR),HES treated group (group HES),HSH treated group(group HSH),shock group(group NR),twelve rats in each group.According to Wiggers method,the rat model of severe hemorrhagic shock was established.MAP was monitored.The level of ALT and AST were tested before the shock,1 h after shock,4 h after resuscitation by taking venous blood.1 h after shock in NR,the rats were killed and the livers were collected.The rats were resuscitated respectively with LR (3 times the volume of shed blood)、HES(1 times the volume of shed blood)and HSH (6 ml/kg).4 hour after resuscitation,the rats in C,LR,HES and HSH group were killed and the livers were collected to determine the expression of TNF-α,NF-κB,MIP-2,MDA,iNOS and activity of MPO,SOD.The pathologic injury of liver was observed by light microscope and electron microscope.Results:Compared with group C,the levels of AST and ALT in NR,LR,HES and HSH groups were significantly higher (P ＜0.05),the expression of NF-κB,TNF-α,MIP-2,iNOS,MDA and activity of MPO,SOD in liver were significantly increased (P ＜0.05),and pathologic injury was increased obiviously.Compared with NR group,the pathologic injury was also increased significant(P＜0.05).Compared with HES group,the cytokines and pathologic injury of LR group was increased significantly(P＜0.05),the cytokines and pathologic injury of HSH group was most lessened (P＜0.05).Conclusion:HSH resuscitation can attenuate the liver injury after severe hemorrhagic shock by decreasing cytokines production,reducing inflammatory reaction and oxygen free radical.关键词
失血性休克/液体复苏/肝损伤/炎症反应/氧自由基Key words
hemorrhagic shock/fluid resuscitation/liver injury/inflammatory reaction/oxygen free radical分类
医药卫生引用本文复制引用
耿智隆,陆华梅,曹虹,杨木强,赵峰..不同液体复苏对重度失血性休克大鼠肝脏损伤的影响[J].西北国防医学杂志,2017,38(12):783-787,5.基金项目
全军“十一五”面上课题项目(06MA083) （06MA083）
军队面上项目(CLZ14L001) （CLZ14L001）
