中国组织工程研究2017，Vol.21Issue(29)：4600-4604,5.DOI:10.3969/j.issn.2095-4344.2017.29.002
甲状旁腺激素对大鼠骨髓间充质干细胞钙离子的影响
Effects of parathyroid hormone on calcium ions in rat bone marrow mesenchymal stem ceils
陈玉书 1张燕红 2张姝江 1白波 1陈艺1
作者信息
- 1. 广州医科大学附属第一医院广东省骨科矫形与植入材料重点实验室,广东省广州市510120
- 2. 南方医科大学顺德医院骨科,广东省顺德市528300
- 折叠
摘要
Abstract
BACKGROUND:Parathyroid hormone can activate a series of physiological and biochemical reactions through specific receptors on the surface of target organs,to adjust the dynamic calcium balance.OBJECTIVE:To explore the mechanisms whereby parathyroid hormone maintains in vitro proliferation of bone marrow mesenchymal stem cells (BMSCs),by observing the effects of parathyroid hormone on calcium ions in BMSCs.METHODS:BMSCs at passage 4 were randomly divided into two groups.Experimental group was treated with parathyroid hormone,and control group was treated with routine culture medium.During 10 days of culture,MTT assay was used to detect BMSCs proliferation.At 7 and 14 days after treatment,laser scanning confocal microscopy was used to detect fluorescence intensity of calcium ions in BMSCs in the two groups.RESULTS AND CONCLUSION:(1) At 1 and 2 days after culture,the cell viability in the two groups was sluggish.At 3 days after culture,the cells turned into the rapid growth.At 7 and 8 days after culture,the cell growth turned into the plateau.At 10 days after culture,the cell proliferation ability had a weakening trend,which was lower in the control group than in the experimental group (P ＜ 0.05).(2) At 7 days after culture,the fluorescence intensity of intracellular calcium ions was strong.As the induction time extended,the fluorescence intensity of intracellular calcium ions was declined at 14 days after-culture.At 7 and 14 days after culture,the fluorescence intensity of intracellular calcium ions in the experimental group was higher than that in the control group (P ＜ 0.001).There was no difference in the fluorescence intensity of intracellular calcium ions between experimental group at 14 days and control group at 7 days.To conclude,parathyroid hormone could increase the fluorescence intensity of calcium ions in BMSCs,and function to maintain the BMSCs proliferation.关键词
干细胞/骨髓干细胞/甲状旁腺激素/钙离子/骨髓间充质干细胞/大鼠/激光共聚焦显微镜分类
医药卫生引用本文复制引用
陈玉书,张燕红,张姝江,白波,陈艺..甲状旁腺激素对大鼠骨髓间充质干细胞钙离子的影响[J].中国组织工程研究,2017,21(29):4600-4604,5.
