中国组织工程研究2017，Vol.21Issue(29)：4605-4610,6.DOI:10.3969/j.issn.2095-4344.2017.29.003
盐酸四环素对大鼠骨髓间充质干细胞的成骨诱导分化活性
Tetracycline hydrochloride induces the osteogenic differentiation of rat bone marrow mesenchymal stem cells
摘要
Abstract
BACKGROUND:Tetracycline hydrochloride not only promotes the proliferation of bone marrow mesenchymal stem cells (BMSCs),but also has a strong bone activity.Studies on the osteogenic differentiation of BMSCs induced by tetracycline hydrochloride will provide a new cell source for bone tissue engineering.OBJECTIVE:To observe the induction and differentiation activity of BMSCs into osteoblasts cultured by tetracycline hydrochloride in vitro.METHODS:BMSCs were separated and expanded by whole bone marrow adherent method and identified by cell morphology and BMSCs cell markers CD34,CD45,CD90,CD29.Passage 3 BMSCs were collected and cultured with tetracycline hydrochloride (experimental group) or cultured in L-DMEM containing 10％ fetal bovine serum (control group).Osteogenic induction and differentiation activity of BMSCs cultured by tetracycline hydrochloride were detected by alkaline phosphatase activity detection,collagen I and osteocalcin immunohistochemistry staining,alizarin red staining of mineralized calcium nodules,and detection of collagen I and osteocalcin gene expression by RT-PCR.RESULTS AND CONCLUSION:Tetracycline hydrochloride continuously promoted alkaline phosphatase activity in the BMSCs,significantly different from that in the control group.After 21 days of culture,positive expression of collagen I and osteocalcin proteins,red calcium nodules shown by alizarin red staining,and mRNA expression of collagen I and osteocalcin were observed in the experimental group,while the negative expression was found in the control group.In conclusion,tetracycline hydrochloride has a significant role to promote the osteogenic differentiation of BMSCs.关键词
干细胞/骨髓干细胞/盐酸四环素/骨髓间充质干细胞/诱导分化/成骨细胞/国家自然科学基金分类
医药卫生引用本文复制引用
张珏,薛斯亮,罗媛,智伟..盐酸四环素对大鼠骨髓间充质干细胞的成骨诱导分化活性[J].中国组织工程研究,2017,21(29):4605-4610,6.基金项目
国家自然科学基金(31400809) （31400809）
贵州省科技厅科学技术基金项目[2012]2366号 （）
中央高校基本科研业务费专项资金(2682015CX006) （2682015CX006）
the National Natural Science Foundation of China,No.31400809 （）
the Science and Technology Foundation of Guizhou Science and Technology Department,No.[2012]2366 （）
the Fundamental Research Funds for the Central Universities,No.2682015CX006 （）
