中国组织工程研究2017，Vol.21Issue(29)：4617-4622,6.DOI:10.3969/j.issn.2095-4344.2017.29.005
人血红素加氧酶1过表达大鼠骨髓间充质干细胞对缺血缺氧环境损伤的耐受能力
Tolerance of rat bone marrow mesenchymal stem cells overexpressing human heme oxygenase 1 to ischemia/hypoxia injury
摘要
Abstract
BACKGROUND:Under ischemia/hypoxia microenvironment,very low survival rate of transplanted bone marrow mesenchymal stem cells (BMSCs) in the host limits its efficacy in the treatment of acute myocardial infarction.OBJECTIVE:To explore the tolerance of human heme oxygenase 1 (hHO-1) gene modified rat BMSCs to ischemia/hypoxia injury.METHODS:The rat BMSCs were transfected with hHO-1 recombinant adenovirus.Western blot assay was used to determinate the optimal time of hHO-1 protein expression.hHO-1 modified rat BMSCs were cultured in hypoxia and serum-free conditions that simulated ischemia/hypoxia microenvironment in vivo.Cell counting kit-8 and trypan blue staining were performed to detect the survival rate of BMSCs at 12,24,48,72 hours after culture under the ischemia/hypoxia microenvironment.Flow cytometry was used to detect apoptosis in BMSCs at 24 hours after culture under the ischemia/hypoxia microenvironment.RESULTS AND CONCLUSION:The expression of hHO-1 protein was highest at 4 days after transfection.Under the ischemia/hypoxia microenvironment for 12,24,48,72 hours,the survival rates of transfected BMSCs were significantly higher as compared with the untransfected cells (P ＜ 0.05),shown by the cell counting kit-8 and trypan blue staining.In addition,the results from flow cytometry showed that there was a higher survival rate of transfected BMSCs than untransfected cells at 24 hours of culture under the ischemia/hypoxia microenvironment (P ＜ 0.05).To conclude,hHO-1 modified rat BMSCs have stronger tolerance to the ischemia/hvpoxia microenvironment.关键词
干细胞/骨髓干细胞/血红素加氧酶1/骨髓间充质干细胞/腺病毒/CCK-8/锥虫蓝/流式细胞术/缺血缺氧/凋亡/国家自然科学基金分类
医药卫生引用本文复制引用
邓宁波,韩腾龙,曾元清,蒋知新..人血红素加氧酶1过表达大鼠骨髓间充质干细胞对缺血缺氧环境损伤的耐受能力[J].中国组织工程研究,2017,21(29):4617-4622,6.基金项目
国家自然科学基金(30971235) （30971235）
军队“十二五”医学科研基金(2013XL010) （2013XL010）
the National Natural Science Foundation of China,No.30971235 （）
the Medical Research Fund of PLA during the Twelfth Five-Year Plan Period,No.2013XL010 （）
