中国组织工程研究2017，Vol.21Issue(29)：4623-4628,6.DOI:10.3969/j.issn.2095-4344.2017.29.006
恒河猴骨髓间充质干细胞的培养与鉴定
Culture and identification of rhesus monkey bone marrow mesenchymal stem cells
摘要
Abstract
BACKGROUND:Studies on rhesus monkey bone marrow mesenchymal stem cells (BMSCs) are limited,and the differentiation potential of juvenile rhesus monkey BMSCs remains unknown.OBJECTIVE:To investigate the biological characteristics of rhesus monkey BMSCs isolated and cultured in vitro,and to identify the growth curve and phenotype of BMSCs.METHODS:BMSCs from juvenile rhesus monkeys were isolated and cultured by density gradient centrifugation and the whole bone marrow adherence methods.BMSCs at passages 2 and 7 were taken to draw cell growth curves by MTS method.Flow cytometry was used to detect apoptosis of passage 3 BMSCs and to detect expression of cell surface markers,CD29,CD34,CD45,CD90,CD147,in passage 4 BMSCs.Osteogenic and adipogenic abilities of passage 3 BMSCs were also detected.RESULTS AND CONCLUSION:The BMSCs at passages 2 and 7 were incubated at first 2 days,logarithmically grew at 3-7 days,and then proliferated slowly at 8 days.The BMSCs at passage 2 showed stronger proliferation than those at passage 7.At passage 3,BMSCs were negative for CD34 and CD45,but positive for CD90,CD29 and CD147.Results from the fiow cytometry showed that there were 73.83％ living cells,21.76％ dead cells,and 4.27％ apoptotic cells.BMSCs could differentiate into osteoblasts and adipocytes under osteogenic and adipogenic induction,respectively.To conclude,BMSCs from the rhesus monkey have strong proliferation and multilineage differentiation abilities in vitro.关键词
干细胞/间充质干细胞/恒河猴/原代培养/形态学/分化/鉴定分类
医药卫生引用本文复制引用
董婧婧,胡竹林,李妍,孙晓梅..恒河猴骨髓间充质干细胞的培养与鉴定[J].中国组织工程研究,2017,21(29):4623-4628,6.基金项目
云南省应用基础研究面上项目(昆医联合专项)(2013FB109,2014FB074):恒河猴自体骨髓间充质干细胞在体诱导为角膜内皮样细胞的实验研究,恒河猴自体骨髓间充质干细胞体外诱导分化为角膜内皮样细胞 （昆医联合专项）
the Applied Basic Research Project of Yunnan Province,No.2013FB109,2014FB074 （）
