西南国防医药2018，Vol.28Issue(1)：16-19,4.DOI:10.3969/j.issn.1004-0188.2018.01.006
新生猕猴UCMSC的制备、鉴定及对老年猕猴自发DM的治疗作用
Preparation and identification of newborn macaque UCMSCs and therapeutic effect on spontaneous DM in senile macaques
摘要
Abstract
Objective To explore the therapeutic effect of umbilical cord-derived mesenchymal stem cells (UCMSCs) from newborn macaques on the spontaneous diabetes mellitus in senile macaques.Methods The tissue explants adherent method was conducted to obtain macaque USMSCs,flow cytometry was used to identify the immunophenotype of UCMSCs,and the in-vitro differentiation capability was analyzed.The UCMSCs were marked with eGFP and then given to 24 w-old macaques with spontaneous DM via intravenous infusion.One month before and after the UCMSC infusion,the levels of serum glucose (Glu),triglyceride (TG),total cholesterol (TC),serum insulin (INS) and serum C-peptide (C-P) were measured in macaques with DM respectively and the changes were compared.Results The isolation and cultivation method of macaque UCMSCs was established,and macaque UCMSCs were obtained;macaque UCMSCs expressed high levels of CD44 and CD29 but failed to express CD34 and CD45,and they could be induced to differentiate into chondrocytes,osteocytes,adipocytes and islet-like cells.After the UCMSC infusion to senile macaques with spontaneous DM,the levels of serum Glu,TG and TC reduced,while the levels of INS and C-P increased.Conclusion Newborn macaque UCMSCs are successfully isolated and proliferated;these UCMSCs have certain therapeutic effect on spontaneous DM in senile macaques.关键词
猕猴/脐带/间充质干细胞/老年/糖尿病/疗效Key words
Macaque/umbilical cord/mesenchymal stem cell/senile/diabetes mellitus/efficacy分类
医药卫生引用本文复制引用
朱向情,王强,蔡学敏,李自安,庞荣清,魏玲,潘兴华..新生猕猴UCMSC的制备、鉴定及对老年猕猴自发DM的治疗作用[J].西南国防医药,2018,28(1):16-19,4.基金项目
昆明市重点项目(2015-1-S-00973) （2015-1-S-00973）
云南省科技计划重点项目(2013CA005) （2013CA005）
