西南国防医药2018，Vol.28Issue(1)：22-25,4.DOI:10.3969/j.issn.1004-0188.2018.01.008
脐带间充质干细胞对CD4+和CD25+Treg细胞的调节作用
Regulatory effect of umbilical cord-derived mesenchymal stem cells on CD4+、CD25+Treg cells
摘要
Abstract
Objective To observe the effect of umbilical cord-derived mesenchymal stem cells (UCMSCs) on CD4+ CD25+ Treg cells in vitro.Mthods Human UCMSCs and lymphocytes were isolated and co-cultured in different proportions in contact and noncontact modes for 72 h;additionally,other lymphocytes were isolated and cultivated to be a control group.Lymphocytes of all groups were collected and treated with the flow cytometry to measure the percentage of CD4+ CD25+Treg cells;and then,the supernate of all groups was obtained and treated with the enzyme-linked immunosorbent assay (ELISA) method to measure the level of IL-10.Results When lymphocytes were co-cultured in contact and non-contact modes with UCMSCs in the proportion of 10:1,Treg cells accounted for the highest percentage among all cells;the levels of CD4+ CD25+Treg cells and IL-10 in all co-culture groups were significantly higher than those in the control group (P ＜ 0.05).Conclusion UCMSCs could induce lymphocytes to differentiate into Treg cells;the mechanism of such differentiation may be related to cytokines released by UCMSCs.关键词
脐带间充质干细胞/CD4+CD25+Treg细胞/白细胞介素10Key words
Umbilical cord-derived mesenchymal stem cell (UCMSC)/CD4+CD25+Treg cell/interleukin-10 (IL-10)分类
医药卫生引用本文复制引用
王强,何洁,赵晶,石琳琳,马丽花,庞荣清,潘兴华..脐带间充质干细胞对CD4+和CD25+Treg细胞的调节作用[J].西南国防医药,2018,28(1):22-25,4.基金项目
云南省科技计划重点项目(2013CA005,2013DA004) （2013CA005,2013DA004）
成都军区“十二五”医学研究重点项目(B12021) （B12021）
