西南国防医药2018，Vol.28Issue(1)：31-35,5.DOI:10.3969/j.issn.1004-0188.2018.01.011
BMSC移植治疗对慢性心衰大鼠心肌结构与功能的影响
Effect of BMSC transplantation therapy on myocardial structure and function of rats with chronic heart failure
摘要
Abstract
Objective To explore the effects of bone marrow-derived mesenchymal stem cell (BMSC) therapy on the myocardial structure and function of rats with chronic heart disease.Methods A total of 30 sprague-dawley (SD) rats were randomly divided into a transplantation group,a model group and a normal control group(n=10,respectively).The transplantation group and the model group were treated with isoproterenol (ISO) via intraperitoneal injection to build the rat model of heart failure.BMSCs were prepared with the whole bone marrow culture method and then transfected and labelled with enhanced green fluorescence protein (EGFP) genes;these EGFP labeled BMSCs were injected through caudal vein into the rats of transplantation group,each rat injected with 5×106 cells/ml.The model group was synchronously injected with equal volume of normal saline,and the normal control group was not treated.Four weeks after the BMSC transplantation,the myocardial tissues were obtained and used to observe the survival and distribution of implanted BMSCs in the tissues;the hematoxylin-eosin (H-E) staining and Masson's trichrome staining were done to show pathological changes such as myocardial fibrosis;the terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) method was conducted to measure the cardiomyocyte apoptosis;the real-time polymerase chain reaction (RT-PCR) technique was used to measure the expression levels of Col Ⅰ mRNA and Col Ⅲ mRNA in myocardial tissues of all groups.Results The Col Ⅰ mRNA and Col ⅢmRNA were expressed in tissues of all groups;the expression levels in the transplantation group were lower than those in the model group (P ＜ 0.05),but were not significantly different from the normal control group (P ＞ 0.05).The correlation analysis showed that the collagen volume fraction (CVF) was inversely correlated with the survival rate of BMSCs.The results of Masson's trichrome staining and TUNEL method showed that the CVF and cardiomyocyte apoptosis rate in the transplantation group and the model group were higher than those in the normal control group (P ＜ 0.01),and those in the transplantation group were lower than those in the model group (P ＜ 0.01).Conclusion BMSCs could inhibit the myocardial fibrosis,the cardiomyocyte apoptosis and the fibrogenesis of collagen,abate the myocardial damage and improve the myocardial structure.关键词
心力衰竭/心肌/胶原/间充质干细胞/心肌纤维化Key words
heart failure/myocardium/collagen/mesenchymal stem cell/myocardial fibrosis分类
医药卫生引用本文复制引用
王欢,朱向情,刘茜,熊云兰,朱姿英,卢国良,潘兴华,魏玲..BMSC移植治疗对慢性心衰大鼠心肌结构与功能的影响[J].西南国防医药,2018,28(1):31-35,5.基金项目
国家科技支撑计划项目(2014BI01B0) （2014BI01B0）
云南省科技计划重点项目(2013CA005) （2013CA005）
