西南国防医药2018，Vol.28Issue(1)：51-53,3.DOI:10.3969/j.issn.1004-0188.2018.01.017
“肺气虚外感”大鼠模型的实验研究
Experimental study on rat model of "deficiency of lung-Qi with exogenous cause"
贺前松 1马萍 1陈瑶1
作者信息
- 1. 610072成都,成都中医药大学基础医学院
- 折叠
摘要
Abstract
Objective To modify and use the classical combination of cigarette smoke+cold air+lipopolysaccharide(LPS) to build a rat model of "deficiency of lung-Qi with exogenous cause" and reduce mechanical injury to and the infection rate of the respiratory duct of model rats.Methods The method "cigarette+cold air stimulation+modified orotracheal injection of LPS" was adopted and the rat model "deficiency of lung-Qi with exogenous cause" was created.During the modeling,the changes in general conditions and weight growth of the model rats were monitored and compared with those of the normal rats.Seven days after the LPS injection,the model rats were killed.The lung,spleen and thymus gland were taken out and weighed.The differences among the indexes of lung,spleen and thymus gland were compared between the model rats and the normal rants;the hematoxylin-eosin (HE) staining was done and then the morphological difference of lungs between the two groups were observed.Retults Five days after the modeling,the general conditions got worse and the weights decreased in the model rat group;the indexes of lung,spleen and thymus gland dropped obviously (P ＜ 0.05-P ＜ 0.01 in each case);the HE staining showed inflammatory cell infiltration and slight sign of emphysema in the lung tissue of the model rat group.Conclion In the method "cigarette smoke +cold air stimulation +modified orotracheal LPS injection",the rat model "deficiency of lung-Qi with exogenous cause" can be successfully established;this method can relieve mechanical injury to the respiratory duct of rat and reduce interference factors that cause post-operative wound infection,and it coincides with the process of pathophysiological evolution of deficiency of lung-Qi with exogenous cause.关键词
肺气虚/外感/大鼠/模型/经口气管注射/脂多糖Key words
deficiency of lung-Qi/exogenous cause/rat/model/orotracheal injection/lipopolysaccharide (LPS)分类
医药卫生引用本文复制引用
贺前松,马萍,陈瑶..“肺气虚外感”大鼠模型的实验研究[J].西南国防医药,2018,28(1):51-53,3.
