烟草科技2017，Vol.50Issue(12)：1-7,7.
印度梨形孢对烟草抗旱性的影响
Effects of Piriformospora indica on drought resistance of Nicotiana tabacum
摘要
Abstract
To investigate the effect of Piriformospora indica on drought resistance of Nicotiana tabacum, a pot experiment was conducted by using polyethylene glycol (PEG) 6000 to simulate drought and the malondialdehyde (MDA) and proline contents, relative electrical conductivity and expression levels of drought-related genes in tobacco leaves under drought stress were analyzed. The results showed that P. indica could colonize in the roots of N. tabacum and promote the growth of both root and shoot of the plants. When P. indica-inoculated plants were under drought stress, the symptoms of water shortage were slighter, the proline content in leaves was significantly up-regulated, while the MDA content and relative electrical conductivity of leaves were significantly decreased comparing with those of un-colonized treatment. Quantitative real-time polymerase chain reaction (qRT-PCR) analysis indicated that the relative expression levels of drought-related genes RD29A, ERD10A, RD26A, SDIR1, HAT, ERD1 and DREB2A were higher in leaves of colonized plants under drought stress. P. indica enhances the drought resistance of N. tabacum via reducing tobacco cell biological membrane damage degree, enhancing the antioxidant ability of tobacco, promoting the accumulation of osmotic substances proline and up-regulating the expression of drought-related genes.关键词
植物内生真菌/印度梨形孢/干旱/抗旱能力/烟草Key words
Plant endophytic fungus/Piriformospora indica/Drought/Drought resistance/Tobacco分类
轻工纺织引用本文复制引用
惠非琼,刘剑,高其康,楼兵干..印度梨形孢对烟草抗旱性的影响[J].烟草科技,2017,50(12):1-7,7.基金项目
贵州中烟工业有限责任公司项目"印度梨形孢对烟草诱导抗病及降低烟叶重金属含量的作用"(201216). （201216）
