烟草科技2017，Vol.50Issue(12)：22-28,7.
牡蛎壳粉对烟草根际土壤微生物代谢多样性及青枯病发生的影响
Effects of oyster shell powder on metabolic diversity of microorganism in tobacco rhizosphere soil and incidence of bacterial wilt
摘要
Abstract
To evaluate the effects of different soil amendments on the metabolic diversity of microorganisms in tobacco rhizosphere soils and the incidence of tobacco bacterial wilt, field experiments were conducted to study the effects of different soil amendments on soil pH and the metabolic diversity of microorganisms and their control efficiencies on tobacco bacterial wilt. Four treatments were set as following: oyster shell powder, quicklime, plant ash and the control. The results showed that oyster shell powder raised soil pH significantly and increased the carbon source utilization by soil microorganisms(Average well color development, AWCD), the metabolic diversity of microorganisms(Shannon index)and the uniformity of species in soil microbial community(McIntosh index). Principal component analysis indicated that carboxylic acids, amino acids and carbohydrates were the main carbon sources utilized by soil microorganisms, however there were some differences in the carbon source utilization characteristics of microorganisms in soils treated by different soil amendments. The incidence of tobacco bacterial wilt in fields treated by oyster shell powder was the lowest,decreased by 43.33% comparing with that of the control. Pearson correlation analysis suggested that soil pH positively correlated to the metabolic diversity index of soil microorganisms, while negatively correlated to disease incidence. Therefore, the application of oyster shell powder can raise soil pH, promote the metabolic diversity of soil microorganisms and the stability of soil micro-ecology environment, and further achieve better control efficiency on tobacco bacterial wilt.关键词
牡蛎壳粉/土壤/改良剂/烟草青枯病/微生物/代谢多样性Key words
Oyster shell powder/Soil/Amendment/Tobacco bacterial wilt/Microorganism/Metabolic diversity分类
农业科技引用本文复制引用
沈桂花,刘晓姣,张淑婷,江其朋,丁伟..牡蛎壳粉对烟草根际土壤微生物代谢多样性及青枯病发生的影响[J].烟草科技,2017,50(12):22-28,7.基金项目
中国烟草总公司科技重点项目"针对烟草青枯病的根际微生态调控防治机制及关键技术研究"(110201502019). （110201502019）
