烟草科技2017，Vol.50Issue(12)：55-60,69,7.
西柏三烯-4,6-二醇降解产香菌培养基条件优化
Optimization of medium for cembratriene-4,6-diol degradation bacteria
摘要
Abstract
In order to improve the degradation rate of cembratriene-4,6-diol, the culture medium for Novosphingobium panipatense YII-3 was optimized by conducting response surface analysis. The results showed that: 1)The optimal carbon source and its concentration, nitrogen source and its concentration, initial pH and cultural temperature for Novosphingobium panipatense YII-3 were confirmed by single factor experiment. 2)The said 4 factors were selected as the response factors, the Box-Behnken response surface analysis of the 4 factors at 3 levels was designed with the degradation rate as the response value. The optimal constituents of the culture medium were determined as maltose 0.66 g/L, yeast extract 0.91 g/L, initial pH 9, and cultural temperature at 35 ℃. 3)Under that condition, the degradation rate increased from 64.58% to 84.71%(or increased by 20.13 percentage points)after optimization. The observed experimental values agreed with the predictive values of mathematic models, and Box-Behnken response surface analysis is suitable for optimizing the culture medium of Novosphingobium panipatense.关键词
西柏三烯-4,6-二醇/新鞘氨醇杆菌/降解率/培养基/Box-Behnken设计/响应面Key words
Cembratriene-4/6-diol/Novosphingobium panipatense/Degradation rate/Culture medium/Box-Behnken design/Response surface分类
轻工纺织引用本文复制引用
黄申,夏璠,钱玉梅,魏涛,贾春晓,毛多斌..西柏三烯-4,6-二醇降解产香菌培养基条件优化[J].烟草科技,2017,50(12):55-60,69,7.基金项目
中国烟草总公司重点实验室项目"西柏烯与胡萝卜素类化合物生物催化降解及应用技术研究"(中烟办[2014] 334号). （中烟办[2014] 334号）
