光学相干断层扫描血管造影(OCTA)在异种角膜移植供体选择中的应用研究
Application of optical coherence tomography angiography in donor selection in xenocorneal transplantation
摘要
Abstract
Objeetive To compare the thickness of corneal epithelium and full-thickness of the cornea among New Zealand rabbits,cats and the human using optical coherence tomography angiography (OCTA) technology as to explore the morphological basis for different animals as experimental models of xenocorneal transplantation.Methods Totally selected 12 New Zealand male rabbits and 12 male cats were included in this study and divided into group A and B.Then the thickness of corneal epithelium and full-thickness of the cornea of both eyes in the two groups were measured with OCTA technology,and according to the distance from the center of the cornea,the cornea was divided into 17 areas by System software.The center of the cornea was a diameter of 2 mm around the center of the cornea,with the inner ring and outer ring of 5 mm and 6 mm in diameter,respectively;and the inner and outer ring region was again divided into 8 areas,including superior side (S),superior nose side (SN),nasal side (N),inferior nose side (IN),inferior side (I),inferior temporal side (IT),temporal side (T),superior temporal side (ST).Then the thickness of corneal epithelium and full-thickness of the cornea in each region were measured for comparing the difference of New Zealand rabbits' and cats' corneal thickness with the human's.Results The thickness of corneal epithelium and full-thickness of the cornea of cats were both thicker than those of rabbits.The full-thickness of the cats' cornea became thinner from the central to the peripheral side,and the difference of thickness between the T5,ST5,S5,SN5,N5 areas and the central of the cornea was statistically significant (all P ＜0.05);the thickness of corneal epithelium in the ST5,S5,SN5 areas was significantly thicker than the central area (all P ＜0.05);And this was true of the rabbits' full-thickness of the cornea,and the difference in the full-thickness of the cornea between the T5,IT5,IN5,N5,T6,N6 area and the central region was statistically significant (all P ＜ 0.05);meanwhile there were also significant difference in the thickness of corneal epithelium between the ST5,S5,SN5,ST6,S6,SN6 areas and the central area (all P ＜ 0.05).Additionally,there were significant differences in the thickness of corneal epithelium and full-thickness of the cornea between the two experimental animals and humans (all P ＜ 0.05).Conclusion The cornea of cats possesses similar regions in corneal full-thickness and epithelial thickness as well as the distribution to human cornea,so cats have superiority to the rabbits for the potential donor of xenocorneal transplantation in human beings.关键词
异种角膜移植/光学相干断层扫描血管造影/兔/猫/角膜上皮Key words
xenocorneal transplantation/optical coherence tomography angiography/rabbit/cat/corneal epithelium分类
医药卫生引用本文复制引用
唐鹏钧,袁晴,马明洋,邵毅,刘启,康红花,韩云,容蓉,申眉,杨启晨,叶蕾,朱佩文..光学相干断层扫描血管造影(OCTA)在异种角膜移植供体选择中的应用研究[J].眼科新进展,2018,38(1):18-22,5.基金项目
国家自然科学基金资助(编号:81660158、81160118、81160148、81460092、81400372、81400424) （编号:81660158、81160118、81160148、81460092、81400372、81400424）
江西省远航工程(编号:2014022) （编号:2014022）
江西省自然科学基金重大项目(编号:2016ACB21017) （编号:2016ACB21017）
江西省青年科学基金(编号:20151BAB215016、20161 BAB215198) （编号:20151BAB215016、20161 BAB215198）
江西省重点研发项目(编号:20151BBG70223) （编号:20151BBG70223）
江西省教育厅重点项目(编号:GJJ160020) （编号:GJJ160020）
江西省教育厅科技计划项目(编号:GJJ160122) （编号:GJJ160122）
江西省卫计委科技计划项目(编号:20164017、20175115、20175116) （编号:20164017、20175115、20175116）
江西省卫计委中医药科技计划项目(编号:20150823).National Natural Science Foundation of China (No:81660158,81 160118,81160148,81460092,81400372,81400424) （编号:20150823）
Jiangxi Province Voyage Project (No:2014022) （No:2014022）
Natural Science Key Project of Jiangxi Province (No:20161ACB21017) （No:20161ACB21017）
Youth Science Foundation of Jiangxi Province (No:20151BAB215016,20161BAB215198) （No:20151BAB215016,20161BAB215198）
Key Research Development Foundation of Jiangxi Province(No:20151BBG70223) （No:20151BBG70223）
Key Education Department Foundation of Jiangxi Province (No:G J J160020) （No:G J J160020）
Education Department Foundation of Jiangxi Province (No:GJJ160122) （No:GJJ160122）
Health Development Planning Commission Science Foundation of Jiangxi Province (No:20164017,20175115,20175116) （No:20164017,20175115,20175116）
Health Development Planning Commission TCM Science Foundation of Jiangxi Province (No:20150823) （No:20150823）
