眼科新进展2018，Vol.38Issue(1)：35-38,4.DOI:10.13389/j.cnki.rao.2018.0007
视网膜神经节细胞无血清上清培养液诱导大鼠视网膜干细胞的分化
Retinal ganglion cell-conditioned medium alters the differentiation of rat retinal progenitor cells
摘要
Abstract
Objective To investigate the effects of retinal ganglion cell-conditioned medium on the differentiation of retinal stem cells.Methods Rettial stem cells and retinal ganglion cells were isolated from rats,and immunofluorescence staining was applied to identify rat retinal stem cells and retinal ganglion cells with Nestin and Thy-1 antibody,respectively.Retinal stem cells were cultured in presence or absence of retinal ganglion cell-conditioned medium for 72 h,followed by detection of Nestin,PAX6,Thy-1 and Bin-3 gene expression in retinal stem cells by qPCR.Results isolated retinal stem cells were Nestin positive,and retinal ganglion cells were Thy-1 positive,indicating the success of isolation.Compared to retinal stem cells cultured without ganglion cellconditioned medium,ones cultured with ganglion-conditioned medium had significantly downregulated expression of Nestin and PAX6 (both P ＜ 0.000 1),and markedly upregulated expression of Thy-1 and Brn-3 (both P ＜ 0.05).Conclusion Retinal ganglion cell-conditioned medium can induce the differentiation of retinal progenitor cells into retinal ganglion-like cells.关键词
视网膜干细胞/视网膜神经节细胞/视网膜神经节细胞无血清上清培养液/Nestin/Thy-1Key words
retinal stem cells/retinal ganglion cells/retinal ganglion cell-conditioned medium/Nestin/Thy-1分类
医药卫生引用本文复制引用
戴敏,张青,郑志坤,沈蔚..视网膜神经节细胞无血清上清培养液诱导大鼠视网膜干细胞的分化[J].眼科新进展,2018,38(1):35-38,4.基金项目
昆医联合专项基金(编号:2014FB075) （编号:2014FB075）
云南省教育厅基金(编号:2014C046Y).Joint Specialized Research Fund from Yunnan Provincial Department of Science and Technology and Kunming Medical University (No:2014FB075) （编号:2014C046Y）
Yunnan Provincial Department of Education Research Fund (No:2014C046Y) （No:2014C046Y）
