摘要
Abstract
ObJective To observe the inhibitory effects of hydrogen on corneal neovascularization (CNV) induced by alkali burn in rats and its underlying mechanisms Methods A total of 48 SPF-grade SD rats (48 eyes) were selected and randomly divided into blank group,control group and treatment group.Then the model of CNV was established successfully induced by alkali burn in these animals.The treatment group was given 1.6 × 10-6 hydrogen eye drops,the control group received tobramycin dexamethasone eye drops,and blank group left untreated.And CNV length was measured on day 1,3,7 and 14 after model establishment,followed by calculation of the CNV area.The corneal and aqueous humor of the rats was removed on day 14 after alkali burn.Then PEDF protein was detected by immunohistochemical staining,and RT-PCR was used to detect the expression of PEDF mRNA in the cornea.Additionally,TNF-α in the aqueous humor was detected by enzyme e-linked immunosorbent assay (ELISA).Resuits The area of corneal neovascularization on day 3,7 and 14 after alkali burn was (3.26 ± 1.82) mm2,(8.59 ± 3.98)mm2 and (3.24 ± 2.34) mm2 in the treatment group,respectively,(4.27 ± 2.68) mm2,(6.44 ± 4.67) mm2 and (15.98 ± 4.75) mm2 in the control group,respectively,(9.49 ±2.79)mm2,(17.71 ±4.06)mm2 and (25.35 ± 1.40)mm2 in blank group,respectively.The CNV areas of the treatment group and the control group were less than that of the blank group on day 3,7 and 14 after alkali bum,and the differences were statistically significant (all P ＜ 0.01),but there was no significant difference between the treatment group and the control group on day 3,7 after alkali bum (P ＞ 0.05),while the area of the treatment group was significantly less than that of the control group on 14th day,and the difference was statistically significant (P ＜0.01).On day 14 after alkali bum,the results of immunohistochemistry showed that the expression of PEDF protein in corneal epithelium was significantly increased in the treatment group than that in the control group and the blank group,and there were few new blood vessels in the stromal layer.RT-PCR results showed that the expression of PEDF mRNA in the treatment group was significantly higher than that in the control group and the blank group,and the difference was statistically significant (all P ＜ 0.01).ELISA results showed that the content of TNF-α protein in the treatment group was significantly lower than that in the control group and the blank group,and the difference was statistically significant (all P ＜0.01).Conclusion 1.6 × 10-6 hydrogen eye drops can effectively inhibit the corneal neovascularization which induced by alkali burn in rats.关键词
氢气水/角膜新生血管/色素上皮衍生因子/肿瘤坏死因子-αKey words
hydrogen/corneal neovascularization/pigment epithelium-derived factor/tumor necrosis factor-α分类
医药卫生
