眼科新进展2018，Vol.38Issue(1)：84-88,5.DOI:10.13389/j.cnki.rao.2018.0019
单细胞转录组测序在眼科研究领域中的应用
Application of single-cell RNA-sequencing in ophthalmology
摘要
Abstract
Single-cell RNA-sequencing (sc RNA-seq) is a new technique allowing the analysis of transcriptomes in individual cell for discovering specific cell type markers,identifying rare cell types and cellular subsets and revealing the different expression among cells.It is becoming an effective method for researching cellular heterogeneity of complex biological system.In recent years,this approach has been widely used in diverse fields of biology,including stem cell,neurobiology,tumor,immunology,microbiology.In addition,it is also applied in ocular research area.As modem molecular biology and bioinformatics develops,there are some commercial platforms emerging gradually with characteristics of high-throughput,low cost and high efficiency.In this review,we will describe some of the current experimental methods used for sc RNA-seq and discuss the merits and drawbacks,as well as summarize the application of this technique in ophthalmology.In the future,this technique may expand to more studies of ocular-associated diseases for the guidance of clinical diagnosis and therapy.关键词
单细胞/RNA测序/转录组/异质性/眼科Key words
single cell/RNA-sequencing/transcriptome/heterogeneity/ophthelmology分类
医药卫生引用本文复制引用
纪艳丽,彭广华..单细胞转录组测序在眼科研究领域中的应用[J].眼科新进展,2018,38(1):84-88,5.基金项目
国家重点基础研究发展计划资助项目(编号:2013CB967001) （编号:2013CB967001）
国家自然科学基金项目(编号:31271400、31470065).National Basic Research Program of China(973 program)(No:2013CB967001) （编号:31271400、31470065）
National Natural Science Foundation of China (No:31271400,31470065) （No:31271400,31470065）
