应用化学2018，Vol.35Issue(1)：28-39,12.DOI:10.11944/j.issn.1000-0518.2018.01.170363
核酸适配体荧光探针在生化分析和生物成像中的研究进展
Aptamer-based Fluorescence Probe for Bioanalysis and Bioimaging
摘要
Abstract
Aptamers are oligonucleotides selected through an in vitro method known as systematic evolution of ligands by exponential enrichment ( SELEX ) , which can recognize target with high specificity and high affinity.Aptamers have attracted wide attention because of their intrinsic advantages including simple synthesis , low molecular mass , high chemical stability , easy biochemical modification and so on .Aptamers can fold into specific two-dimensional or three-dimensional configurations and specifically bind with their targets.With appropriate signal transduction mechanism , aptamers can provide ideal molecular recognition and detection probes for biological research .Fluorescence is a type of detection technology that has several superiorities of high sensitivity , high resolution and easy multiplex detection .With the combination of the molecular recognition capability of aptamers and the outstanding detection performance of fluorescence technology , the aptamer-based fluorescence probes have been widely used in the study of life science .This review highlights the major progress of aptamer-based fluorescence probes in both bioanalysis and bioimaging area, and discusses the current limitations of aptamer probe for applications .关键词
核酸适配体/荧光探针/生化分析/生物成像Key words
aptamer/fluorescence probe/bioanalysis/bioimaging分类
化学化工引用本文复制引用
黄子珂,刘超,付强强,李进,邹建梅,谢斯滔,邱丽萍..核酸适配体荧光探针在生化分析和生物成像中的研究进展[J].应用化学,2018,35(1):28-39,12.基金项目
国家自然科学基金青年科学基金项目(21505039) 资助 Supported by the National Natural Science Foundation of China(No.21505039) （21505039）
