中国感染控制杂志2017，Vol.16Issue(11)：993-998,6.DOI:10.3969/j.issn.1671-9638.2017.11.001
不同细菌所致小鼠血流感染模型中MIP-1β、MIP-2和IL-12p70的表达及变化规律
Expression and variation of MIP-1β, MIP-2, and IL-12p70 in mouse models with bloodstream infection caused by different bacteria
摘要
Abstract
Objective To investigate the expression and variation of MIP 1β,MIP-2,and IL-12p70 in mice with bloodstream infection caused by 4 kinds of bacteria.Methods CD-1 (ICR) mouse models of bloodstream infection with Staphylococcus aureus (S.aureus),Enterococcus f aecalis (E.f aecalis),Escherichia coli (E.coli),and K lebsiella pneumoniae (K.pneumoniae) were established.After mice in each trial group and PBS control group were infected by bacteria for 0.5h,1h,3h,6h,12h,24h,and 48h,concentrations of MIP-1β,MIP-2,and IL-12p70 were detected by Luminex liquid suspension chip system.Results Concentrations of MIP-1β increased significantly 1h after bacteria was in blood,S.aureus,E.faecalis,E.coli,K.pneumoniae,and control groups were (134.5 ± 18.3),(61.5 ± 15.4),(3 354.0 ±809.0),(6 888.4 ± 1 100.2),and (28.9 ± 4.6) pg/mL respectively;the peak values of IL-12p70 were (389.3 ± 118.1),(127.6 ± 10.0),(42.2 ± 3.5),(62.8 ± 8.4),and (4.8 ± 0.3) pg/mL respectively.Concentrations of MIP-1β and MIP-2 in E.coli and K.pneumoniae groups were significantly higher than other trial groups and control group (all P＜0.01),while concentrations of IL-12p70 in S.aureus and E.faecalis groups were both significantly higher than E.coli,K.pneumoniae,and control groups (all P＜0.01).Conclusion Concentrations of MIP-1β and MIP-2 in E.coli and K.pneumoniae groups were both significantly higher than those in S.aureus and E.faecalis groups,while concentrations of IL-12p70 in S.aureus and E.faecalis groups were both significantly higher than those in E.coli and K.pneumoniae groups.The combination detection of multiple cytokines or chemokines are valuable in predicting gram-positive or gram-negative bacterial infection,and can provide basis for treatment of early infection.关键词
血流感染/MIP-1β/MIP-2/小鼠模型/IL-12p70Key words
bloodstream infection/MIP-1β/MIP-2/mouse model/IL-12p70分类
医药卫生引用本文复制引用
杨明,麻雅婷,何赏,段欣欣,王佳楠,荆颖,张可昕,王成彬..不同细菌所致小鼠血流感染模型中MIP-1β、MIP-2和IL-12p70的表达及变化规律[J].中国感染控制杂志,2017,16(11):993-998,6.基金项目
国家重大科学仪器专项项目任务(2012YQ18011708) （2012YQ18011708）
