中国康复理论与实践2018，Vol.24Issue(1)：11-16,6.DOI:10.3969/j.issn.1006-9771.2018.01.003
环磷酸腺苷效应元件结合蛋白/脑源性神经营养因子信号通路在调心方改善APP/PS1小鼠学习记忆中的作用
Role of cAMP Response Element Binding Protein/Brain-derived Neurotrophic Factor Signaling Pathway in Tiaoxin Formula Improving Learning and Memory of APP/PS1 Mice
摘要
Abstract
Objective To observe the changes of cAMP response element binding protein (CREB)/brain-derived neurotrophic factor (BDNF) signaling pathway in the hippocampus of APP/PS1 double transgenic Alzheimer's disease mice, and in-vestigate the effects of Tiaoxin formula on learning and memory. Methods Totally, 54 three-month-old APP/PS1 transgenic mice were randomly divided into model group (n=18), posi-tive control (donepezil) group (n=18) and Tiaoxin formula group (n=18). Another 18 three-month-old C57/BL6 wild mice were chosen as normal group with same strain and same age. All administration groups received rele-vant medicine. Twelve weeks later, Morris water maze test was used to test the behavior. The real-time quantita-tive polymerase chain reaction (RT-qPCR) was used to detect the expression of CREB mRNA and BDNF mRNA in hippocampus. Western blotting was used to detect the expression of phosphorylated CREB (p- CREB) and BDNF in hippocampus. Results Compared with the model group, the escape latency significantly shortened (P<0.01), the percentage of staying time in the quadrant of original platform significantly increased (P<0.05), the expression of CREB mRNA and BDNF mRNA in hippocampus significantly increased (P<0.05), and the expression of p-CREB and BDNF pro-tein in hippocampus significantly increased (P<0.01) in Tiaoxin formula group. Conclusion Tiaoxin formula could improve the spatial learning and memory of APP/PS1 double transgenic mice with AD, which might be related to the up-regulation of CREB/BDNF signaling pathway.关键词
阿尔茨海默病/调心方/Morris水迷宫/学习记忆/环磷酸腺苷效应元件结合蛋白/脑源性神经营养因子/信号通路/小鼠Key words
Alzheimer's disease/Tiaoxin formula/Morris water maze/learning and memory/cAMP response element binding protein/brain-derived neurotrophic factor/signaling pathway/mice分类
医药卫生引用本文复制引用
王晓雯,邓海燕,王健,徐颖..环磷酸腺苷效应元件结合蛋白/脑源性神经营养因子信号通路在调心方改善APP/PS1小鼠学习记忆中的作用[J].中国康复理论与实践,2018,24(1):11-16,6.基金项目
上海市自然科学基金项目(No. 13ZR1439700) Supported by Shanghai Municipal Natural Science Foundation (No. 13ZR1439700) （No. 13ZR1439700）
