中国康复理论与实践2018，Vol.24Issue(1)：17-22,6.DOI:10.3969/j.issn.1006-9771.2018.01.004
不同电针法对APP/PS1小鼠海马高迁移率族蛋白B1和白细胞介素-10表达的影响
Effects of Different Types of Electroacupuncture on Expression of High Mobility Group Box Pro-tein-1 and Interleukin-10 in Hippocampus of APP/PS1 Mice
摘要
Abstract
Objective To compare the effect of impulse electroacupuncture (impulse-EA) and music electroacupuncture (music-EA) for Tongdu Qishen on spatial learning and memory, and expression of cytokine in the hippocampus of APP/PS1 mice. Methods A total of 32 APP/PS1 double transgenic male mice were randomly divided into model group (n=8), drug group (n=8), impulse-EA group (n=8) and music-EA group (n=8), the same background and age male C57BL/6 mice were observed as normal group (n=8). The impulse-EA group and music-EA group accepted EA at Baihui (GV20) and Yintang (GV29), connected with their own electroacupuncture stimulators, for 20 minuts, then, they were pricked Renzhong (GV26) for a while. The drug group accepted donepezil hydrochloride 0.92 mg/kg intra-gastrically. The normal group, model group and drug group were grabbed and bounded in the same way. After 15 days of treatment, they were assessed with Morris water maze. The expression of high mobility group box pro-tein-1 (HMGB1) and interleukin-10 (IL-10) in hippocampus were measured with immunohistochemistry and Western blotting. Results The escape latency shortened inEA groups compared with that of the model group since the fourth day of Morris water maze training (P<0.05), and it was the least in the music-EA group; and the ratio of swimming across the target quadrant increased (P<0.05), but there was no significant difference between EA groups (P>0.05). The ex-pression of HMGB1 decreased (P<0.05) and the expression of IL-10 increased (P<0.05) in EA groups com-pared with that of the model group, and HMGB1 decreased more and IL-10 increased more in the music-EA group than in the impulse-EA group (P<0.05), according to the measurement in immunohistochemistry, not in Western blotting. Conclusion Both impulse-EA and music-EA based on Tongdu Qishen can promote the recovery of the learning and memory in APP/PS1 mice, and music-EA may do more effectively in inhibition of pro-inflammatory factors and promotion of the anti-inflammatory factors.关键词
阿尔茨海默病/电针/学习记忆/高迁移率族蛋白B1/白细胞介素-10/小鼠Key words
Alzheimer's disease/electroacupuncture/learning and memory/high mobility group box protein-1/interleu-kin-10/mice分类
医药卫生引用本文复制引用
赵江豪,姚海江,王远征,卢梦晗,李昱颉,宋萌,杨利娟,刘俊彤,李志刚..不同电针法对APP/PS1小鼠海马高迁移率族蛋白B1和白细胞介素-10表达的影响[J].中国康复理论与实践,2018,24(1):17-22,6.基金项目
国家自然科学基金面上项目(No. 81473774) Supported by National Natural Science Foundation of China (General) (No. 81473774) （No. 81473774）
