中国康复理论与实践2018，Vol.24Issue(1)：23-28,6.DOI:10.3969/j.issn.1006-9771.2018.01.005
雷公藤内酯醇对阿尔茨海默病大鼠海马drebrin和cofilin表达的影响
Effects of Triptolide on Expression of Drebrin and Cofilin in Hippocampus of Rats with Alzheim-er's Disease
摘要
Abstract
Objective To observe the effects of triptolide on drebrin and cofilin expression in the hippocampus of rats with Alzheim-er's disease (AD). Methods Sixty male Sprague-Dawley rats were equally divided into control group, model group and triptolide-treated group with 20 cases in each group. The AD model was established with unilateral injection of beta amyloid 1-40 (Aβ1- 40) into hippocampus in rats. The control group was established with unilateral injection of normal saline with the same volume into hippocampus in rats. The triptolide-treated group was administered triptolide intraperi-toneally, 0.4 mg/kg, once a day, for 15 days after modeling. Spine density of hippocampal neurons was assayed by Golgi staining. Drebrin and cofilin expression of hippocampal neurons was assayed by immunohistochemical staining and reverse transcription polymerase chain reaction (RT-PCR). Results The spine density of hippocampal neurons was higher in the triptolide-treated group than in the model group (P<0.05). The average optical density of drebrin was higher in the triptolide-treated group than in the the model group (P<0.01), while the cell number and average optical density of cofilin were lower (P<0.05). The drebrin mRNA expression was higher in the triptolide-treated group than in the model group (P<0.05), and the cofilin mRNA expression was lower (P<0.01). Conclusion Triptolide may delay the degeneration of dendritic spines in hippocampal neurons of AD rats by regulating the expression of drebrin and cofilin.关键词
阿尔茨海默病/雷公藤内酯醇/drebrin/cofilin/大鼠Key words
Alzheimer's disease/triptolide/drebrin/cofilin/rats分类
医药卫生引用本文复制引用
张赛圣,杨宝林,程丽霞,万斌,聂菁,胡小令,吕诚..雷公藤内酯醇对阿尔茨海默病大鼠海马drebrin和cofilin表达的影响[J].中国康复理论与实践,2018,24(1):23-28,6.基金项目
1.国家自然科学基金项目(No. 81660191 （）
No. 30660073) （）
2. 江西省教育厅科研基金项目(No. GJJ150105) （No. GJJ150105）
3. 江西省卫生计生委科技计划项目(No. 20165532) Supported by National Natural Science Foundation of China (No. 81660191 （No. 20165532）
No. 30660073), Jiangxi Education De-partment Research Fund (No. GJJ150105) and Jiangxi Health and Family Planning Commission Science and Technolo-gy Project (No. 20165532) （No. GJJ150105）
