中国康复理论与实践2018，Vol.24Issue(1)：37-42,6.DOI:10.3969/j.issn.1006-9771.2018.01.007
鱼藤酮对MN9D细胞蛋白磷酸酶2A活力的影响
Influence of Rotenone on Activity of Protein Phosphates 2A in MN9D Cells
摘要
Abstract
Objective To study whether and how rotenone reduces the activity of protein phophatase 2A (PP2A). Methods MN9D cells (mouse midbrain dopaminergic cell line) were divided into normal group (normal cultured), con-trol group (dimethyl sulfoxide of same volume with rotenone group was added in medium), rotenone group (50 nmol/L rotenone was added to the culture medium for 24 hours) and rotenone+C2 group (pretreatment of 5 mol/L C2-ceramide for eight hours, and then exposed to 50 nmol/L rotenone for 24 hours). MTT was used to detect cell viability. Total PP2A levels and tyrosine phosphorylation levels were measured with Western blotting. PP2A activity was detected with colorimetric assay.Results Compared with the control group, the cell viability reduced (P<0.01), phosphorylation of tyrosine 307 of PP2A inceased (P<0.01), and activity of PP2A decreased in the rotenone group (P<0.05). And compared with the rote-none group, the cell viability improved (P<0.01), phosphorylation of tyrosine 307 of PP2A deceased (P<0.01), and activity of PP2A increased (P<0.05) in the rotenone+C2 group. Conclusion Rotenone can inhibit activity of PP2A through increasing phosphorylation of tyrosine 307 at the catalytic subunit of PP2A, which might be involved in reducing cell viability, and implicate a new treatment strategy for Parkinson's disease.关键词
帕金森病/鱼藤酮/细胞活力/蛋白磷酸酶2AKey words
Parkinson's disease/rotenone/cell viability/protein phosphatase 2A分类
医药卫生引用本文复制引用
王屹,杜婷婷,刘舒佳,刘佳,卢勇泉,杨慧..鱼藤酮对MN9D细胞蛋白磷酸酶2A活力的影响[J].中国康复理论与实践,2018,24(1):37-42,6.基金项目
1.中国康复研究中心科研专项基金项目(No. 2017ZX-26) （No. 2017ZX-26）
2. 国家重点研究发展计划项目(No. 2016YFC1306002) Supported by China Rehabilitation Research Center Project (No. 2017ZX-26) and National Key R&D Plan of China (No. 2016YFC1306002) （No. 2016YFC1306002）
