中国康复理论与实践2018，Vol.24Issue(1)：43-48,6.DOI:10.3969/j.issn.1006-9771.2018.01.008
电针百会、神庭穴对缺血再灌注损伤大鼠学习记忆能力及海马CA1区嘌呤受体P2X7表达的影响
Effects of Electroacupuncture at Baihui and Shenting on Learning and Memory and Expression of Purinoceptor P2X7 in Hippocampal CA1 after Cerebral Ischmeia-reperfusion in Rats
摘要
Abstract
Objective To observe the effect of electroacupuncture at Baihui (GV20) and Shenting (GV24) on learning and memory in rats after cerebral ischmeia-reperfusion and the possible mechanism. Methods A total of 42 male Sprague-Dawley rats were randomly divided into sham group (n=12) and operation group (n=30). The left middle cerebral arteries of the operation group were occluded with the modified Longa's method for 90 minutes and reperfused, and 24 qualified rats were randomly divided into model group (n=12) and elec-troacupuncture group (n=12), and the latter accepted electroacupuncture at Baihui and Shenting for seven days. They were assessed with Longa's score two hours after modeling, and one, three, seven days after intervention. They were tested with Barnes maze since three days after intervention, once a day for five days. The expression of purinoceptor P2X7 in CA1 of the hippocampus were detected with immunofluorescence seven days after inter-vention, while the expression of interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α) in CA1 were detect-ed with enzyme-linked immunosorbent assay. Results The Longa's score was improved in the electroacupuncture group compared with that in the model group seven days after intervention (P<0.05); while the escape latency and the times entering the wrong hole increased in the model group compared with that in the sham group (P<0.001), and decreased in the electroacupuncture group compared with that in the model group (P<0.001). The expression of P2X7, IL-1β and TNF-α increased in the model group compared with the sham operation group (P<0.001), and decreased in the electroacupuncture group compared with that in the model group (P<0.05). Conclusion Electroacupuncture at Baihui and Shenting can improve the learning and memory in rats after cerebral isch-emia-reperfusion, which may associate with inhibition of P2X7 to alleviate inflammation in hippocampus.关键词
脑缺血再灌注/电针/嘌呤受体/P2X7/海马/学习记忆/炎症/大鼠Key words
cerebral ischemia-reperfusion/electroacupuncture/purinoceptor/P2X7/hippocampus/learning and memo-ry/inflammation/rats分类
医药卫生引用本文复制引用
黄佳,宋长明,杨敏光,李建鸿,黄盛,柳维林,陶静,陈立典..电针百会、神庭穴对缺血再灌注损伤大鼠学习记忆能力及海马CA1区嘌呤受体P2X7表达的影响[J].中国康复理论与实践,2018,24(1):43-48,6.基金项目
1.国家自然科学基金项目(No. 81403462) （No. 81403462）
2.福建省卫生厅青年科研项目(No. 2014-1-70) Supported by National Natural Science Foundation of China (No. 81403462) and Youth Research Project of Fujian Health Department (No. 2014-1-70) （No. 2014-1-70）
