中国康复理论与实践2018，Vol.24Issue(1)：49-53,5.DOI:10.3969/j.issn.1006-9771.2018.01.009
电针神庭、百会对脑缺血再灌注大鼠认知功能及 Beclin-1表达的影响
Effect of Electroacupuncture on Learning and Memory and Expression of Beclin-1 in Rats after Cerebral Ischemic-reperfusion
摘要
Abstract
Objective To observe the efficacy of electroacupuncture at Baihui (GV20) and Shenting (GV24) on learning and memo-ry in rats after cerebral ischemia-reperfusion, and explore the possible mechanism. Methods A total of 45 male Sprague-Dawley rats were randomly divided into sham group (n=15), ischemia group (n=15) and electroacupuncture group (n=15). The left middle cerebral arteries of the latter two groups were occluded with the modified Longa's method for two hours and reperfused, and the electroacupuncture group received elec-troacupuncture at Baihui and Shenting once a day for seven days, then. They were assessed with Longa's score one, three, five and seven days after intervention. They were tested with Morris water maze since four days after intervention, once a day for four days. The cerebral infarction volume was measured with TTC staining seven days after intervention, while the protein expression of Beclin-1 was detected with Western blotting. Results Longa's score improved in the electroacupuncture group compared with that in the ischemia group since three days after intervention (P<0.05). The escape latency decreased and the times cross the area of platform increased in the electroacupuncture group compared with that in the ischemia group at every point (P<0.05). The cerebral infarction volume reduced (F=7.651, P<0.001) and the expression of Beclin-1 decreased (P<0.05) in the elec-troacupuncture group compared with that in the ischemia group.Conclusion Electroacupuncture can improve learning and memory in rats after cerebral ischemia-reperfusion, which may relate with regulation of autophagy network.关键词
脑缺血再灌注/电针/学习记忆/自噬/Beclin-1/大鼠Key words
cerebral ischemia-reperfusion/electroacupuncture/learning and memory/autophagy/Beclin-1/rats分类
医药卫生引用本文复制引用
冯晓东,史景,湾明月,刘承梅..电针神庭、百会对脑缺血再灌注大鼠认知功能及 Beclin-1表达的影响[J].中国康复理论与实践,2018,24(1):49-53,5.基金项目
国家自然科学基金项目(No. 14202214) Supported by National Natural Science Foundation of China (No. 14202214) （No. 14202214）
