中国康复理论与实践2018，Vol.24Issue(1)：54-59,6.DOI:10.3969/j.issn.1006-9771.2018.01.010
电针预处理对脑缺血再灌注大鼠缺血半暗区细胞凋亡及凋亡相关蛋白表达的影响
Effects of Electroacupuncture Preconditioning on Apoptosis and Expression of Apoptosis-related Proteins in Rats after Cerebral Ischemia-reperfusion
摘要
Abstract
Objective To investigate the effect of electroacupuncture (EA) preconditioning on apoptosis after cerebral ischemia-re-perfusion (I/R). Methods A total of 72 male Sprague-Dawley rats were randomly divided into sham group (n=24), model group (n=24) and EA group (n=24). The rats in latter two groups were occluded the right middle cerebral arteries for two hours and reperfused. EA group was treated with EA at Baihui (GV20) for two weeks before modeling. They were as-sessed with modified Neurological Severity Scores (mNSS) 24 hours after modeling. Then, the cerebral infarct volume was measured with TTC staining, the apoptosis was detected with TUENL assay, and the expression of p53, Bax and Bcl-2 proteins in ischemic penumbra was detected with Western blotting. Results Compared with the model group, the score of mNSS, cerebral infarct volume and the number of TUNEL-posi-tive cells all significantly decreased (P<0.05) in EA group; while the expression of p53 and Bax proteins de-creased (P<0.05), Bcl-2 increased (P<0.05), and Bax/Bcl-2 decreased (P<0.05).Conclusion EA preconditioning can induce tolerance to cerebral I/R injury, which might associate with the inhibition of p53 protein and down-regulation of the Bax/Bcl-2 ratio in ischemic penumbra, to inhibit cerebral cell apoptosis.关键词
脑缺血再灌注/电针/预处理/凋亡/p53/Bax/Bcl-2/大鼠Key words
cerebral ischemia-reperfusion/electroacupuncture/preconditioning/apoptosis/p53/Bax/Bcl-2/rats分类
医药卫生引用本文复制引用
叶涛,朱路文,唐强,李宏玉,吴孝军,陈晨,姜云飞,李佳帅..电针预处理对脑缺血再灌注大鼠缺血半暗区细胞凋亡及凋亡相关蛋白表达的影响[J].中国康复理论与实践,2018,24(1):54-59,6.基金项目
1.国家自然科学基金项目(No. 81503666) （No. 81503666）
2.黑龙江省自然科学基金项目(No. QC2015103) （No. QC2015103）
3.黑龙江省普通本科高等学校青年创新人才培养计划项目(No. 201680) Supported by National Natural Science Foundation of China (No. 81503666), Heilongjiang Natural Science Founda-tion (No. QC2015103) and Heilongjiang Program for Distinguished Young Talents in Higher Education (No. 201680) （No. 201680）
