中国康复理论与实践2018，Vol.24Issue(1)：66-70,5.DOI:10.3969/j.issn.1006-9771.2018.01.012
沉默白细胞介素-1β基因对脊髓钝挫伤大鼠波形蛋白的影响
Effect of Interleukin-1β Silence on Expression of Vimentin in Rats with Spinal Cord Contusion
摘要
Abstract
Objective To observe the expression of vimentin (Vim) after silence of interleukin-1β (IL-1β) in rats with spinal cord contusion (SCC). Methods The model of SCC was established in 30 Sprague-Dawley rats with Allen's method. The rats were randomized into vector group (n=15) and silence group (n=15), which were injected blank lentivirus vector and vector of IL-1β siRNA, respectively; and divided in three, seven and 28 days subgroups. The relationship between IL-1βand Vim was predicted with GeneMANIA bioinformatics. The expression of Vim protein and mRNA in spinal cord was detected with immunohistochemistry and real-time quantitative polymerase chain reaction. Results GeneMANIA bioinformatic analysis indicated that there was some direct and indirect relationship between IL-1β and Vim. The Vim protein and mRNA expressed in the spinal cord, and was less in the silence group than in the vector group (t>2.875, P<0.05). Conclusion Silence of IL-1β can inhibit the expression of Vim in SCC rats, which may promote the recovery of spinal cord function.关键词
脊髓钝挫伤/白细胞介素-1β/波形蛋白/siRNA/大鼠Key words
spinal cord contusion/interleukin-1β/vimentin/siRNA/rats分类
医药卫生引用本文复制引用
胡析,牛英杰,黄媛,李盈洁,曾茜,向阳,张晓,梁楠..沉默白细胞介素-1β基因对脊髓钝挫伤大鼠波形蛋白的影响[J].中国康复理论与实践,2018,24(1):66-70,5.基金项目
1.国家级大学生创新创业训练计划项目(No. 201613705028) （No. 201613705028）
2.四川省大学生创新创业训练计划项目(No. 201613705059) Supported by National Students' Innovation and Entrepreneurship Training Program (No. 201613705028) and Sich-uan Province Students' Innovation and Entrepreneurship Training Program (No. 201613705059) （No. 201613705059）
