中国水产科学2018，Vol.25Issue(1)：1-8,8.DOI:10.3724/SP.J.1118.2018.17125
利用蛋白抗体芯片筛选盐胁迫下红罗非鱼鳃差异表达蛋白
Screening the differentially expressed proteins by protein chip in gill of red tilapia (Oreochromis mossambicus♀×O.niloticus♂) under sa-linity stress
摘要
Abstract
Due to its rapid growth rate and excellent adaptability to saline environments, the red tilapia (Oreo-chromis mossambicus♀×O. niloticus♂)could be a suitable subject for studies on saline tolerance.In this study, to provide a theoretical basis for saline tolerance mechanisms, we aimed to screen and identify differentially ex-pressed proteins in the gill of Red tilapia using protein chips and mass spectrometry, which were subsequently verified by using immunohistochemistry and western blot techniques. There were 181 differentially expressed protein spots detected (change multiple ≥ 1.5), which included 142 up-regulated proteins and 39 down-regulated proteins. Among these, three proteins were identified by mass spectrometry from five differentially expressed proteins: intermediate filament protein (IF), translocation protein 63 (SEC63), and disulfide-isomerase A3 (PDIA3). The immunohistochemistry results showed that IF and PDIA3 are expressed in the gill base of red tilapia under both freshwater and saline water conditions, and showed a trend of initial decrease and then increase with an increase of saline concentration. However, no positive reaction was detected for SEC63. The western blot results showed IF and PDIA3 expression level changes in different saline waters similar to those observed using im-munohistochemistry. In the 30 g/L salinity group, the expression of IF protein decreased during the early stage of stress, reached the lowest value at 48 h, and thereafter began to rise significantly at 72 h, The expression of PDIA3 protein was significantly increased after 96 h (P < 0.05). These results suggest that IF and PDIA3 are positively responsive proteins in the gill tissues of red tilapia under salinity stress, and play important roles in the regulation of cytoskeleton and endoplasmic reticulum function.关键词
红罗非鱼/鳃/盐胁迫/差异表达蛋白/蛋白质芯片Key words
Oreochromis mossambicus×O.niloticus/gill/salinity stress/differentially expressed proteins/protein chip分类
农业科技引用本文复制引用
黄思颖,赵金良,王燕,赵永华,涂翰卿,赵岩..利用蛋白抗体芯片筛选盐胁迫下红罗非鱼鳃差异表达蛋白[J].中国水产科学,2018,25(1):1-8,8.基金项目
国家现代农业产业技术体系建设专项(CARS-46) （CARS-46）
水产动物遗传育种中心上海市协同创新中心(ZF1206). （ZF1206）
