中国水产科学2018，Vol.25Issue(1)：74-85,12.DOI:10.3724/SP.J.1118.2018.17187
饥饿及再投喂处理对草鱼生长、葡萄糖代谢和转运蛋白1表达的影响
Effects of starvation and refeeding on growth performance, glucose metabolism,and expression of glucose transporter 1 in Ctenopharyn-godon idellus
摘要
Abstract
This experiment was conducted to determine the effects of starvation and refeeding on the growth per-formance, biochemical indices in the plasma, liver/muscle glycogen content, glucose metabolic enzymes, and gene expression of glucose transporter 1 (GLUT1) in grass carp (Ctenopharyngodon idellus) and then clarify the char-acteristics of glucose metabolism of grass carp under starvation and refeeding. The combined experiment consisted of a starvation group (S), refeeding group (R, starvation 4 weeks + refeeding 4 weeks), and continuous feeding group (C). Fish with an average initial weight of (125.35±0.54) g were randomly selected in each treatment and injected with 300 mg glucose per 100 g body weight for a glucose tolerance test (GTT) at the end of experiment. The results showed that starvation resulted in a low liver glycogen content, plasma glucose, and triglyceride con-centration. However, starvation treatment had a significant effect on glucose tolerance, and the plasma glucose concentration of the S group was significantly lower (P<0.05) than that of the other groups during the entire sam-pling period, whereas liver glycogen content peaked at 6 h. Starvation treatment had a significant effect on activi-ties of carbohydrate metabolic enzymes. Starvation induced the liver PEPCK enzyme activity but depressed PK and PFK enzyme activities. However, PEPCK, PK, and PFK enzyme activities returned to their previous levels after refeeding. After glucose injection, a significant increase (P<0.05) in GK and PK activities was observed in the S group, but PK activities of the R group significantly increased (P<0.05) and then decreased. Starvation treatment had a significant effect on the expression of GLUT1 in hepatic and muscle tissues.After the glucose load, starvation and refeeding significantly induced the expression of GLUT1 in hepatic and muscle tissues (P<0.05);however,GLUT1 expression in the muscle tissue of the R group decreased, whereas its expression in the muscle tissue of the S group was significantly higher than that of the other groups during the entire sampling period (P<0.05). It could be concluded that during the stages of starvation, to deal with the challenges of food deprivation, grass carp depleted glycogen and triglyceride, suppressed hepatic glycolysis enzymes, such as PK and PFK, and activated hepatic glycogenolytic enzymes, such as PEPCK. The results of starvation treatment in grass carp fa-vored the improvement of glucose tolerance through inducing GK and PK enzyme activities, increasing the syn-thesis of liver glycogen,and activating the expression of GLUT1 in hepatic and muscle tissues.关键词
草鱼/饥饿及再投喂/急性高糖胁迫/糖代谢/葡萄糖转运蛋白1Key words
Ctenopharyngodon idellus/starvation and refeeding/acute hyperglycemia stress/glucose metabolism/glucose transporter 1分类
农业科技引用本文复制引用
李锐鑫,刘泓宇,谭北平,董晓慧,迟淑艳,杨奇慧,章双..饥饿及再投喂处理对草鱼生长、葡萄糖代谢和转运蛋白1表达的影响[J].中国水产科学,2018,25(1):74-85,12.基金项目
国家自然科学基金项目(31272673) （31272673）
国家973重点基础研究计划项目(2014CB138603) （2014CB138603）
广东省珠江学者基金项目(GDUPS 2011) （GDUPS 2011）
广东省自然科学基金项目(S2013010016511) （S2013010016511）
湛江市科技攻关项目(2016A03011). （2016A03011）
