中国水产科学2018，Vol.25Issue(1)：195-203,9.DOI:10.3724/SP.J.1118.2018.17046
嗜水气单胞菌灭活疫苗免疫施氏鲟后的免疫应答反应与保护效果
Immune responses and protective efficacy in Amur sturgeon(Acipenser schrenckii) after immunization with formalin-killed Aeromonas hy-drophila
摘要
Abstract
In order to study the immune responses and protective efficacy in Amur sturgeon(Acipenser schrenckii Brandt, 1869), we used formalin-killed Aeromonas hydrophila to immunize healthy Amur sturgeon by peritoneal injection. We investigated the following immune parameters: peripheral blood cell count, differential leucocyte count, phagocytic activity and index, acid phosphatase activity, lysozyme activity, neutralizing antibody, and rela-tive percentage survival. The numbers of erythrocytes and leucocytes in the peripheral blood of immunized fish increased rapidly and reached maximum values of (8.50 ± 0.17) × 108/mL and (8.96 ± 0.44) × 106/mL, respectively, at 4 d post-immunization. The percentages of monocytes and neutrophils reached maximum values of 10.50% and 15.53%, respectively, at 4 d post-immunization, and were very significantly higher than those of fish in the control groups (P < 0.01). However, lymphocyte percentage reached a maximum value of 73.51% at 21 d post-immuniz-ation. The phagocytic index and percentage of neutrophils were 4.53 and 30%, respectively, at 4 d post-immunization. The activities of lysozyme and acid phosphatase in serum reached peaks at 7 d and 14 d post-immunization, respectively. Neutralizing antibodies in the immunized fish reached 1 : 203 at 21 d post-immunization. When the immunized fish were challenged with live A.hydrophila, the relative percentage survival of the immunized group was 76.84%. These results showed that the formalin-killed A.hydrophila was able to elicit immune protection in Amur sturgeon, which lays the foundation for the further research on im-munoprophylaxis against the diseases caused by A.hydrophila infection in sturgeons.关键词
嗜水气单胞菌/施氏鲟/灭活疫苗/免疫指标/相对免疫保护率Key words
Aeromonas hydrophila/Amur sturgeon/inactivated vaccine/immune index/relative percentage sur-vival分类
农业科技引用本文复制引用
周勇,史玉恒,赵建青,代建丽,范玉顶,刘文枝,江南,曾令兵..嗜水气单胞菌灭活疫苗免疫施氏鲟后的免疫应答反应与保护效果[J].中国水产科学,2018,25(1):195-203,9.基金项目
国家973计划项目(2015CB150703). （2015CB150703）
