中国现代医学杂志2017，Vol.27Issue(25)：13-18,6.DOI:10.3969/j.issn.1005-8982.2017.25.003
性别差异对磷脂转运蛋白基因敲除小鼠胆固醇逆转运效率的影响
Effect of gender difference on reverse cholesterol transport efficiency in PLTP-/-mice
摘要
Abstract
Objective To compare the effects of phospholipid transfer protein gene knockout (PLTP-/-) on reverse cholesterol transport effiency,and observe the changes of plasma lipids in male and female PLTP-/-mice.Methods In this study 8 male and 8 female 8-week littermate PLTP-/-mice were selected and fed with high-fat diet for 4 weeks.Then plasma lipids such as total cholesterol (TC),triglyceride (TG),high-density lipoprotein cholesterol (HDL-C) and non-high density cholesterol (non-HDL-C) were detected by an enzymatic method.The plasma level of apolipoprotein A1 (apoA1) was analyzed by Western blot.Then all mice were injected intraperitoneally with 3H-cholesterol labeled macrophages and the appearance of 3H-tracer in plasma,liver,bile,intestinal wall and feces over 48 h was determined by liquid scintillation counter.The mRNA expressions of scavenger receptor class B type 1 (SR-B1),low density lipoprotein-receptor (LDL-R),ATP-binding cassette transporter G5/G8 (ABCG5/G8),liver X receptor α (LXRα) and cholesterol 7α-hydroxylase A1 (CYP7A1) in liver,and ABCG5/G8 and LXRα in intestinal wall were determined by RT-PCR.Results Compared with the male PLTP-/-mice,the expressions of TC,TG,HDL-C and non-HDL-C in the female PLTP-/-mice were not obvious changed,the expression of apoA1 was increased significantly (P ＜ 0.05).The isotope tracing experiment showed 3H-cholesterol of plasma and intestine had no differences between the female and male mice;3H-tracer of liver,bile and feces was increased significantly (P ＜0.05).Meanwhile,RT-PCR analysis showed the mRNA expressions of LXRα,ABCG5/G8 and CYP7A1 in the liver were up-regulated significantly in the female mice compared with the male mice (P ＜ 0.05),while the mRNA expressions of SRB1 and LDL-R in the liver and LXRα and ABCG5/G8 in the intestinal wall were not different between the female and male mice (P ＞ 0.05).Conclusions Reverse cholesterol transport efficiency is higher and the mRNA expressions of the genes related to reverse cholesterol transport in liver are also significantly higher in the female PLTP-/-mice than in the male PLTP-/-mice.关键词
磷脂转运蛋白/胆固醇逆转运/3H-胆固醇/性别差异Key words
phospholipid transfer protein/reverse cholesterol transport/3H-cholesterol/gender difference分类
医药卫生引用本文复制引用
张颖,司艳红,秦树存..性别差异对磷脂转运蛋白基因敲除小鼠胆固醇逆转运效率的影响[J].中国现代医学杂志,2017,27(25):13-18,6.基金项目
山东省自然科学基金(No:ZR2013HL063) （No:ZR2013HL063）
山东省泰山学者岗项目(No:ts20151105) （No:ts20151105）
