中国现代医学杂志2017，Vol.27Issue(26)：7-12,6.DOI:10.3969/j.issn.1005-8982.2017.26.002
大气颗粒物PM10对小鼠泪膜功能和角膜上皮组织结构的影响
Influence of air pollutant PM10 on tear film and corneal epithelium in mice
摘要
Abstract
Objective To investigate the influence of PM10 on tear film functions and corneal epithelium in mice.Method Totally 30 male BALB/c mice at the age of 6-8 weeks (30 eyes) were divided into 3 groups:group A (PBS eye drops,n=12),group B (PM1o eye drops,n=12) and blank control group (n =6).The group B used 5 mg/ml PM10 eye drops,50 μl each time,3 times a day,for 7 consecutive days.The group A used sterile PBS as eye drops,3 times a day,for 7 consecutive days.The tear film function tests including tear secretion level,breakup time of tear film (BUT) and fluorescein staining and fluorescein staining score (FL) were performed before therapy and 1,4 and 7 d after treatment.On the 7th d after treatment,the corneas of all the mice were collected for HE staining and ultrastructural examination under transmission electron microscope.Results On the 1st,4th and 7th d after treatment,there was no statistical change in tear secretion level,BUT or FL in the group A (P＞ 0.05);but there were statistical changes in all items in the group B (P ＜ 0.05).On the 7th d after therapy,the mean layers of corneal epithelial cells in the group A were significantly fewer than those in the group B (P ＜ 0.05).Under electron microscope,the microvilli of corneal epithelium in the group A were finger-like and in regular arrangement;in contrast,a cluster of disordered,shorter and fewer microvilli was observed in the group B.Conclusions PM10 can influence tear film functions and damage corneal epithelial structure in mice.关键词
PM10/泪膜功能/角膜上皮Key words
particular matter 10/tear film function/corneal epithelium分类
医药卫生引用本文复制引用
李娟,谭钢,吴安花,杨启晨,叶蕾,王亚虹,邵毅..大气颗粒物PM10对小鼠泪膜功能和角膜上皮组织结构的影响[J].中国现代医学杂志,2017,27(26):7-12,6.基金项目
国家自然科学基金(No:81400424) （No:81400424）
陕西省科学技术研究发展计划(No:2014K 11-03-07-04) （No:2014K 11-03-07-04）
2017年陕西省创新人才推进计划-青年科技新星项目(No:2017KJXX-87) （No:2017KJXX-87）
