中国现代医学杂志2017，Vol.27Issue(26)：13-17,5.DOI:10.3969/j.issn.1005-8982.2017.26.003
TGF-β1、BMP-7及Gremlin在小鼠肝纤维化组织中的表达
Expressions of TGF-β1, BMP-7 and Gremlin in CCl4-induced liver fibrosis tissue of mice
摘要
Abstract
Objective To study the expressions of transforming growth factor-beta 1 (TGF-β1),bone morphogenetic protein 7 (BMP-7) and Gremlin in the liver fibrosis induced by carbon tetrachloride (CCl4) in mice,and to investigate their roles in the pathogenesis of hepatic fibrosis.Methods Forty male C57 mice were randomly divided into control group and experimental group.In the experimental group,acute liver injury of mice was induced by intraperitoneal injection of 40％ CCl4 olive oil solution,10 ml/kg,while the control group was injected with olive oil,twice a week for 8 weeks.After 8 weeks the mice were sacrificed,and the liver tissue was fixed.Hematoxylin-eosin (HE) staining was used to observe the degree of liver fibrosis.The expressions and distribution of TGF-β1,BMP-7 and Gremlin in the control and experimental groups were detected through immunohistochemical staining.qR-PCR detecting system and Western blot were utilized for detection of mRNA and protein expressions of TGF-β1,BMP-7 and Gremlin in the normal and fibrotic liver tissues.Results In the experimental group,both mRNA and protein expressions of TGF-β1 and Gremlin in the liver fibrosis tissue were significantly enhanced (P＜ 0.05).However,the expression of BMP-7 in the liver fibrosis tissue was significantly decreased (P＜ 0.05).The expression levels of TGF-β1 and Gremlin were positively related to hepatic fibrosis,while BMP-7 was negatively correlated to hepatic fibrosis (P＜ 0.05).Conclusions Gremlin may induce the occurrence of liver fibrosis by down-regulation of BMP-7 expression.关键词
转化生长因子β1/骨形态发生蛋白7/Gremlin/肝纤维化Key words
TGF-β1/BMP-7/Gremlin/hepatic fibrosis分类
医药卫生引用本文复制引用
徐靖宇,帅张丽,谢远,龙从风,石国庆..TGF-β1、BMP-7及Gremlin在小鼠肝纤维化组织中的表达[J].中国现代医学杂志,2017,27(26):13-17,5.基金项目
贵州省科学技术基金[No:黔科合J字(2012)2359号] （2012）
