中国现代医学杂志2017，Vol.27Issue(26)：30-34,5.DOI:10.3969/j.issn.1005-8982.2017.26.006
鞣质联合环丙沙星对鲍曼不动杆菌生物膜形成的影响
Tannin combination with Ciprofloxacin could effect on biofilm of Acinetobacter baumanni
摘要
Abstract
Objective To explore the effect of tannin extracted from Pomegranate rind combined with Ciprofloxacin (CIP) on the biofilm of Acinetobacter baumanni (Ab).Methods The minimal inhibitory concentrations (MIC) of tannin and CIP to Ab ATCC19606 were determined by broth dilution method.The 1/2MIC,MIC and 2MIC of tannin,CIP and their combination acted on Ab for 4,8,24 and 48 h,respectively;then the biofilm formation of Ab was observed by crystal violet staining assay and fluorescence microscopy.Results The MIC of tannin and CIP to Ab ATCC19606 were 1.95 mg/ml and 0.50 mg/ml respectively.The effect of tannin or CIP separately on the biofilm of Ab was weaker than that of their combination.And their combination could have some effect on the biofilms,of which the combined use of 2MIC tannin and 2MIC CIP for 24 h had the greatest effect on biofilm formation of Ab.Conclusions Combined use of tannin extracted from Pomegranate rind and CIP has more obvious effect on the biofilm formation of Ab,moreover,the combination of 2MIC tannin and 2MIC CIP for 24 h has the best effect.关键词
鲍曼不动杆菌/生物膜/石榴皮提取物鞣质/环丙沙星Key words
Acinetobacter baumanni/biofilm/tannin extracted from Pomegranate rind/Ciprofloxacin分类
医药卫生引用本文复制引用
彭丹,宋丽,陈泽慧,董泽令,周小仙,陈安林,李亚男..鞣质联合环丙沙星对鲍曼不动杆菌生物膜形成的影响[J].中国现代医学杂志,2017,27(26):30-34,5.基金项目
贵州省科技厅合作计划[No:黔科合LH(2015)7480)] （2015）
遵义医学院基金[No:院字(2009)22号] （2009）
