中国现代医学杂志2017，Vol.27Issue(26)：35-39,5.DOI:10.3969/j.issn.1005-8982.2017.26.007
亚抑菌浓度庆大霉素对铜绿假单胞菌的影响
Effet of sub-minimal inhibitory concentration of Gentamycin on Pseudomonas aeruginosa
杨正贵 1季艳艳 1何照庆1
作者信息
- 1. 贵州盘江投资控股(集团)有限公司总医院检验科,贵州六盘水553536
- 折叠
摘要
Abstract
Objective To study the effect of Gentamycin of sub-minimal inhibitory concentration (sub-MIC) on Pseudomonas aeruginosa.Methods Microdilution method was used to detect the sensitivity of P.aeruginosa to Gentamycin.And the turbidity method was used to check the effect of sub-MIC of Gentamycin on the growth of P.aeruginosa planktonic bacteria.Then,crystal violet method was used to explore the effect of sub-MIC Gentamycin on the biofilm formation of P.aeruginosa.Finally,chemical colorimetric method was utilized to test the effect of sub-MIC Gentamycin on the virulence factor production of P.aeruginosa.Results The MIC of Gentamycin on P.aeruginosa was 2 μg/ml.Gentamycin ≤0.250 μg/ml had no influence on the growth of planktonic bacteria.Gentamycin could significantly enhance the biofilm formation at the concentration of 0.125-0.250 μg/ml and in a dose-dependent manner (P＜0.05).At the same time,0.125 and 0.250 μg/ml Gentamycin could significantly promote the expressions of the quorum sensing system (QS) related genes lasI,lasR,rhlI,rhlR,pqsC and pqsD,and the virulence factors pyocyanin,elastase and alkaline protease in a dose-dependent manner (P＜ 0.05).Conclusions sub-MIC Gentamycin could enhance the biofilm formation and the expressions of QS related genes and virulence factors.关键词
庆大霉素/压抑菌浓度/铜绿假单胞菌/生物膜/青脓素/群体密度感应Key words
sub-MIC/Gentamycin/Pseudomonas aeruginosa/biofilm/pyocyanin/quorum sensing分类
医药卫生引用本文复制引用
杨正贵,季艳艳,何照庆..亚抑菌浓度庆大霉素对铜绿假单胞菌的影响[J].中国现代医学杂志,2017,27(26):35-39,5.
