中国现代医学杂志2017，Vol.27Issue(26)：45-51,7.DOI:10.3969/j.issn.1005-8982.2017.26.009
循环型microRNA-26a及其靶基因PTEN与系统性红斑狼疮的相关性研究
Association of circulating miR-26a and its target gene PTEN with systemic lupus erythematosus
崔丹瑜 1朱丁季 1任昊 1林静丽 1赖伟男 1黄琴 1赵进军 1杨敏1
作者信息
- 1. 南方医科大学附属南方医院风湿免疫科,广东广州510515
- 折叠
摘要
Abstract
Objective To explore the expressions of circulating miR-26a and its target gene PTEN in systemic lupus erythematosus (SLE) and their possible roles in the occurrence and development of SLE.Methods The expressions of circulating miR-26a and PTEN in peripheral blood mononuclear cells (PBMCs)were detected by qRT-PCR after blood samples were collected from 36 cases of SLE and 10 healthy people.The correlations between miR-26a,PTEN and clinical data of SLE were statistically analyzed.Dual luciferase reporter system was used to test whether PTEN was the target gene of miR-26a.Results The expression of miR-26a mRNA in the serum of the SLE patients was higher than that in the healthy people (P＜ 0.05),and the expression of PTEN mRNA in the PBMCs of the SLE patients was lower than that in the PBMCs of the healthy people (P ＜ 0.05).The expression levels of miR-26a and PTEN in the patients with SLE were correlated with C3,anti-dsDNA,ESR and SLEDAI.After miR-26a was transfected to BaF3 cells,the expression of PTEN was down-regulated,and the expression of PI3K was up-regulated,and the luciferase activity of the transfected group was decreased (P ＜ 0.05).Conclusions The over-expression of circulating miR-26a may participate in the occurrence and development of SLE through down-regulation of its target gene PTEN and up-regulation of PI3K/AKT signaling pathway.关键词
系统性红斑狼疮/循环型microRNA-26a/PTEN/PI3K/AKTKey words
systemic lupus erythematosus/circulating miR-26a/PTEN/PI3K/AKT分类
医药卫生引用本文复制引用
崔丹瑜,朱丁季,任昊,林静丽,赖伟男,黄琴,赵进军,杨敏..循环型microRNA-26a及其靶基因PTEN与系统性红斑狼疮的相关性研究[J].中国现代医学杂志,2017,27(26):45-51,7.
