中国现代医学杂志2018，Vol.28Issue(3)：1-5,5.DOI:10.3969/j.issn.1005-8982.2018.03.001
冬虫夏草对糖尿病肾病大鼠肾小管细胞中AMPK/mTOR信号传导途径的影响
Cordyceps sinensis inhibits autophagy through AMPK / mTOR signaling pathway in rat renal tubular cell model
摘要
Abstract
Objective To investigate the effect of cordyceps sinensis (CS) on regulating autophagy in rat renal tubular cells model. Methods Streptozotocin was injected intraperitoneally to establish rat model of diabetic nephropathy (DN), and 60 rats were randomly divided into control group, model group and experimental group. Rats were sacrificed 8 weeks post strptozotocin injection. Urine protein and serum creatinine were quantified. HE staining was performed to observe renal pathological abnormality. TUNEL analysis was utilized to determine apoptosis rate of renal tubular cell. Expression levels of p-AMPK, p-mTOR and LC-II were measured by Western blot. Results Physical as well as biochemical abnormalities in model group was significantly attenuated by CS treatment (P < 0.05). HE staining suggested that streptozotocin-induced renal tubular injury and renal tubular epithelial cell shedding death were ameliorated dramatically by CS. Apoptosis rate of renal tubular epithelial cells in experimental group was significantly decreased when compared with that in model group (P < 0.05). Conclusion CS preserves renal function by inhibition of AMPK / mTOR signaling pathway-mediated autophagy in renal tubular epithelial cells.关键词
冬虫夏草/糖尿病肾病/肾小管上皮细胞/AMPK/MtorKey words
Cordyceps sinensis/diabetic nephropathy/renal tubular epithelial cells/AMPK / Mtor分类
医药卫生引用本文复制引用
徐喆,赵凯,李志军..冬虫夏草对糖尿病肾病大鼠肾小管细胞中AMPK/mTOR信号传导途径的影响[J].中国现代医学杂志,2018,28(3):1-5,5.基金项目
国家自然科学基金青年科学基金项目(No:81503409) （No:81503409）
