中国现代医学杂志2018，Vol.28Issue(3)：6-9,4.DOI:10.3969/j.issn.1005-8982.2018.03.002
硫氧还蛋白相互作用蛋白在大鼠急性肾损伤中的作用
Role of thioredoxin-interacting protein in rat model of acute renal injury
摘要
Abstract
Objective To investigate the role of thioredoxin-interacting protein (TXNIP) in rat model of acute renal injury. Methods Renal ischemia reperfusion (IR) model was established by bilateral occlusion of renal pedicle for 25 min followed by 48 hours' reperfusion. Totally 16 male Sprague-Dawley rats were randomly divided into 2 groups (n = 8): Sham (S) group and IR group. Kidneys were harvested at the 48th h post reperfusion. HE staining was performed for morphological abnormalities. Blood urea nitrogen (BUN) and Serum creatinine (Scr) were measured by fully automatic biochemical analyzer. Concentration of Superoxide Dismutase (SOD) and Malondialdehyde (MDA) were determined by colorimetry. Cellular apoptosis rate was identified by TdT-mediated dUTP Nick-End Labeling. Expression levels of TXNIP, Nod-like receptor protein 3 (NLRP3) and IL-1β were determined by Western blot or Enzyme-linked immunosorbent assay (ELISA). Results Morphological abnormalities such as tissue swelling, interstitial edema, loss of brush border and the necrotic cavitation in renal tubules of IR group were observed. Levels of BUN, Scr, MDA, ,TXNIP, NLRP3, IL-1β as well as cellular apoptosis rate in IR group increased significantly when compared with Sham group (P < 0.05), while concentration of SOD decreased in IR group in comparison with Sham group (P < 0.05). Conclusion TXNIP is involved in renal ischemia reperfusion injury by activing NLRP3/IL-1β inflammatory pathway.关键词
硫氧还蛋白相互作用蛋白/炎性体3蛋白/炎症通路/急性肾损伤Key words
TXNIP/NLRP3/inflammatory pathway/acute kidney injury分类
医药卫生引用本文复制引用
汪华新,肖业达,赵博,唐朝亮,黄亚医,詹丽英,高文蔚..硫氧还蛋白相互作用蛋白在大鼠急性肾损伤中的作用[J].中国现代医学杂志,2018,28(3):6-9,4.基金项目
湖北省自然科学基金(No:2016CFB167) （No:2016CFB167）
中央高校基本科研业务费专项基金(No:2042017kf0144) （No:2042017kf0144）
