中国现代医学杂志2018，Vol.28Issue(3)：10-15,6.DOI:10.3969/j.issn.1005-8982.2018.03.003
大鼠压疮局部不同温度治疗对PI3K/Akt/GSK3β 信号通路的影响
Effect of different local temperature on PI3K/Akt/GSK3β pathway in rat model of pressure ulcer
摘要
Abstract
Objectives To investigate the effect of different local temperature on PI3K/Akt/GSK3β pathway in rat model of pressure ulcer. Methods The rat model of pressure ulcer was established by ischemia reperfusion (IR) method. 40 SD rats of SPF grade were divided into Sham group, NTI group, HTI group and LTI group. HE staining was performed to observe pathologies of skeletal muscle. Western blot analysis was utilized to determine the phosphorylation of Akt and GSK3β. Expression levels of p-Akt and p-GSK3β were determined by Immunofluorescence; TUNEL staining was performed to identify apoptosis rate of skeletal muscle. Results Morphological abnormalities of skeletal muscle, apoptosis rate increased while phosphorylation level of Akt and GSK3β decreased in HTI group when compared with NTI group (P < 0.05). In contrast, low temperature can restrain the skeletal muscle cell damage and apoptosis, and increased the phosphorylation of Akt and GSK3β when compared with NTI group (P < 0.05). Conclusions Local hypothermia can enhance recovery of pressure ulcer injury by increasing the phosphorylation of Akt and GSK3β, and this can provide experimental evidence for clinical usage of hypothermia for treatment of pressure ulcer.关键词
温度/压疮/缺血再灌注损伤/PI3K/Akt/GSK3β信号通路Key words
temperature/pressure ulcer/ischemia reperfusion injury/PI3K / Akt / GSK3β pathway分类
医药卫生引用本文复制引用
王晴,白燕平,邢凤梅,汪凤兰,张小丽..大鼠压疮局部不同温度治疗对PI3K/Akt/GSK3β 信号通路的影响[J].中国现代医学杂志,2018,28(3):10-15,6.基金项目
华北理工大学研究生创新项目(No:2017S30) （No:2017S30）
