中国现代医学杂志2018，Vol.28Issue(3)：16-20,5.DOI:10.3969/j.issn.1005-8982.2018.03.004
低氧诱导因子-1α 转染对骨髓间充质干细胞与β-磷酸三钙支架相容性的影响研究
Effect of HIF-1α expression on compatibility of MSCs with β-TCP scaffolds
摘要
Abstract
Objective To investigate the effect of hypoxia inducible factor-1α (HIF-1α) on compatibility of bone marrow mesenchymal stem cells (MSCs) with beta tricalcium phosphate (β-TCP) scaffold. Methods The primary MSCs were isolated by direct adherent method. Flow cytometry was performed for validation of MSCs as well as its differential capacity to osteoblast. HIF-1α was transfected into the third generation (P3) of MSCs by liposome. HIF-1α positive cells were screened by geneticin 418 (G418). β-TCP scaffold plus MSCs was divided into 2 groups: group A in which MSCs and β-TCP scaffolds were co-cultured, group B in which HIF-1α positive MSCs and β-TCP were co-cultured. The survival rate and growth curve of MSCs were observed. Result MSCs grew well on the β-TCP scaffolds prior and post HIF-1α transfection, maintaining differential capacity without statistical significance (P >0.05). No significant difference was observed between the two groups in cell number and cell proliferation (P > 0.05). Conclusion HIF-1α expression does not affect the growth and proliferation of MSCs on β-TCP scaffold.关键词
低氧诱导因子-1α/骨髓间充质干细胞/转染/β-磷酸三钙支架Key words
hypoxia inducible factor-1α/mesenchymal stem cells/transfection/beta tricalcium phosphate分类
生物科学引用本文复制引用
张扬,李学渊,牟怡平..低氧诱导因子-1α 转染对骨髓间充质干细胞与β-磷酸三钙支架相容性的影响研究[J].中国现代医学杂志,2018,28(3):16-20,5.基金项目
沈阳医学院科技基金(No:20155047) （No:20155047）
辽宁省教育厅科技一般项目(No:L2013316) （No:L2013316）
